Controlled stirred tank bioreactors for large-scale manufacture of human iPSC models for cell therapy

Langenberg, Kerstin U.; Kamphorst, Jurre J.; Bonhomme, C.; D’Amico, Eugenio; Dublin, S.; Braam, Stefan; Reijerkerk, Arie

doi:10.1016/j.jcyt.2020.03.042

Cited by 6 publications

(1 citation statement)

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“…However, there are changes in the tumorigenic conversion of these cells, which is a threat during the treatment regimen [ 163 ]. However, the use of induced pluripotent stem cells (iPSCs) in the generation of NK cells might be a future strategy to resolve these issues and to obtain large-scale production of NK cells for therapies [ 164 , 165 ]. Another problem is the identification of an appropriate tumor antigen for developing CAR-NK cells that target resistant tumor cells [ 162 ].…”

Section: Limitations Of Nanotechnology-based Nk Cell Therapymentioning

confidence: 99%

Nanoparticle Enhancement of Natural Killer (NK) Cell-Based Immunotherapy

Murugan

Murugesan

Panchapakesan

et al. 2022

Cancers

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Natural killer (NK) cells are one of the first lines of defense against infections and malignancies. NK cell-based immunotherapies are emerging as an alternative to T cell-based immunotherapies. Preclinical and clinical studies of NK cell-based immunotherapies have given promising results in the past few decades for hematologic malignancies. Despite these achievements, NK cell-based immunotherapies have limitations, such as limited performance/low therapeutic efficiency in solid tumors, the short lifespan of NK cells, limited specificity of adoptive transfer and genetic modification, NK cell rejection by the patient’s immune system, insignificant infiltration of NK cells into the tumor microenvironment (TME), and the expensive nature of the treatment. Nanotechnology could potentially assist with the activation, proliferation, near-real time imaging, and enhancement of NK cell cytotoxic activity by guiding their function, analyzing their performance in near-real time, and improving immunotherapeutic efficiency. This paper reviews the role of NK cells, their mechanism of action in killing tumor cells, and the receptors which could serve as potential targets for signaling. Specifically, we have reviewed five different areas of nanotechnology that could enhance immunotherapy efficiency: nanoparticle-assisted immunomodulation to enhance NK cell activity, nanoparticles enhancing homing of NK cells, nanoparticle delivery of RNAi to enhance NK cell activity, genetic modulation of NK cells based on nanoparticles, and nanoparticle activation of NKG2D, which is the master regulator of all NK cell responses.

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