Controlled biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) from mixtures of palm kernel oil and 3HV-precursors

Bhubalan, Kesaven; Lee, Wing-Hin; Loo, Ching-Yee; Yamamoto, Tetsuya; Tsuge, Takeharu; Doi, Yoshiharu; Sudesh, Kumar

doi:10.1016/j.polymdegradstab.2007.11.004

Cited by 103 publications

(48 citation statements)

References 22 publications

(30 reference statements)

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“…By co-feeding soybean oil and c-butyrolactone as C sources, P(3HB:4HB) can be synthesized by R. eutropha KCTC2662 having 4HB fractions of 6-10 mol% [22]. Tercopolymers P(3HB:3HB:3HHx) was reported from palm kernel oil mixed with 3HV precursors [58,59].…”

Section: Substrate and Feeding Regimementioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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Section: Substrate and Feeding Regimementioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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“…However, in this range of high 3HV content, incorporation of longer chain monomers have shown to drastically decrease the melting enthalpy compared to 3HB-3HV copolymers. For a terpolymer of 3HB-3HV-3HHx (38:60:2 mol%), Bhubalan et al (2008) observed a melting enthalpy of 7.6 J/g and for a terpolymer containing 3-hydroxyheptanoate, 3HHp (with 42:48:10 mol% 3HB:3HV:3HHp), Fukui et al (1997) observed a melting enthalpy of 17 J/g. For polymers containing 3HB, 3H2MB, 3HV and 3H2MV with 79 mol% and 90 mol% non-3HB, Dai et al (2008) observed melting enthalpies from 9.4 J/g to 20 J/g.…”

Section: Glass Transition and Melting Propertiesmentioning

confidence: 99%

Molecular weight and thermal properties of polyhydroxyalkanoates produced from fermented sugar molasses by open mixed cultures

Bengtsson¹,

Pisco

Johansson³

et al. 2010

Journal of Biotechnology

123

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“…Polymers containing monomers greater than C 6 are usually made by the second pathway (Aldor and Keasling 2003). As a result, production of C 6 units typically requires hexanoate, octanoate, or longer-chain-length fatty acids (Bhubalan et al 2008;Chen et al 2001;Doi et al 1995). When short-chain precursors or substrates such as acetate, propionate, and butyrate were used for PHA production in Ralstonia eutropha H16, it resulted in the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(HB-co-HV)), and butyrate (C 4 ) was not used in R. eutropha H16 for the production of poly(3-hydroxybutryrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) (Yang et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha

Jeon

Brigham

Kim

et al. 2014

Appl Microbiol Biotechnol

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Polyhydroxyalkanoates (PHAs), a promising family of bio-based polymers, are considered to be alternatives to traditional petroleum-based plastics. Copolymers like poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) have been shown to exhibit favorable physical and mechanical properties, due to decreased crystallinity resulting from the presence of medium-chain-length 3-hydroxyhexanoate (3HHx) monomers. In this study, we produced P(HB-coHHx) using engineered Ralstonia eutropha strains containing deletions of the acetoacetyl-CoA reductase (phaB) genes and replacing the native PHA synthase with phaC2 from Rhodococcus aetherivorans I24 and by using butyrate, a short-chain organic acid, as the carbon source. Although the wild-type R. eutropha did not produce P(HB-co-HHx) when grown on mixed acids or on butyrate as the sole carbon source, we are able to produce polymer containing up to 40 wt% 3HHx monomer with the aforementioned engineered R. eutropha strains using various concentrations of just butyrate as the sole carbon source. This is the first report for the production of P(HB-co-HHx) copolymer in R. eutropha using butyrate.

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Controlled biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) from mixtures of palm kernel oil and 3HV-precursors

Cited by 103 publications

References 22 publications

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Molecular weight and thermal properties of polyhydroxyalkanoates produced from fermented sugar molasses by open mixed cultures

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha

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