Controllable Cell Deformation Using Acoustic Streaming for Membrane Permeability Modulation

Guo, Xinyi; Sun, Mengjie; Yang, Yang; Xu, Hui; Ji, Liu; He, Song; Wang, Yanyan; Xu, Lianyong; Pang, Wei; Duan, Xuexin

doi:10.1002/advs.202002489

Cited by 45 publications

(37 citation statements)

References 49 publications

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“…Figure 4h,i illustrate non-bubble-based sonoporation mechanisms that rely on acoustic streaming. As shown in Figure 4h, hyper-frequency (>1 GHz) [173][174][175][176][177][178] bulk acoustic waves generated from a GHz acoustic resonator can generate intense acoustic streaming, which applies a shear force on a cell adhered on a substrate. Studies show that the applied shear force is strong enough to deform the cell and enhance the membrane permeability.…”

Section: Non-bubble-based Mechanismsmentioning

confidence: 99%

“…Studies show that the applied shear force is strong enough to deform the cell and enhance the membrane permeability. [178] On the other hand, surface acoustic waves generated by an interdigital transducer (e.g., tapered or focused transducer) can also enable strong surface acoustic wave streaming (Figure 4i) and, therefore, apply a strong shear force to enhance the membrane permeability. [179,180] In addition to the aforementioned non-bubble-based mechanisms, the acoustic energy dissipation can lead to thermal energy, influencing the sonoporation performance.…”

Section: Non-bubble-based Mechanismsmentioning

confidence: 99%

“…The hyper-frequency bulk acoustic waves are generated by GHz acoustic resonators, which are nanofabricated multilayered structures with thin electrodes, a piezoelectric layer, and reflector layers (see Figure 7a,b). [173,177,178] Guo et al found that the generated hyper-frequency acoustic waves could lead to strong acoustic streaming, which further applied shear forces on cells attached on a substrate to change the membrane stress and induce cell deformation. [178] Because of the membrane stress change, their technology can increase the membrane permeability for intracellular delivery.…”

Section: Hyper-frequency Bulk Acoustic Wave-based Streaming Technologiesmentioning

confidence: 99%

“…[173,177,178] Guo et al found that the generated hyper-frequency acoustic waves could lead to strong acoustic streaming, which further applied shear forces on cells attached on a substrate to change the membrane stress and induce cell deformation. [178] Because of the membrane stress change, their technology can increase the membrane permeability for intracellular delivery. By periodically applying hyper-frequency acoustic waves (1.64 GHz frequency and 50% duty cycle) for 10 min, the GHz acoustic resonators can successfully deliver high-molecular-weight dextran (40 kDa) to cancer cells adhered on a substrate (Figure 7c) with the delivery efficiency of ≈47% and viability of ≈70%.…”

Section: Hyper-frequency Bulk Acoustic Wave-based Streaming Technologiesmentioning

confidence: 99%

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Sonoporation: Past, Present, and Future

Rich

Tian

Huang

2021

Adv Materials Technologies

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only contribute to fundamental studies in areas of biology, bioengineering, and medicine, but also benefit several life-changing technologies, such as cell-based therapies, protein therapeutics, vaccines, as well as the reprogramming of cells. [22] Among the current delivery approaches, the viral vector-based approach is often considered the gold standard for the delivery of nucleic acids. [22] However, due to notable weaknesses such as biohazards, inconsistent results, and labor-intensive manufacturing, [22][23][24] physical permeabilization approaches are being pursued, [1][2][3][4][5][6][7][8][9][10][11][12]22] including electroporation, [25,26] hydroporation, [27][28][29][30][31][32] mechanoporation, [25,[32][33][34][35][36][37][38][39][40] and sonoporation. [41,42] These approaches are noted as advanced due to their high throughputs, cost-effectiveness, and universal nature to deliver a variety of cargos regardless of cell type. [3] Of the physical permeabilization approaches, sonoporation wields great potential and has been demonstrated as an efficacious technology for delivering a variety of functional cargos, such as biomolecular drugs, proteins, and plasmids, to different types of cells, such as cancer, immune, and stem cells. [43][44][45][46][47][48] Sonoporation was discovered in the 1980s, a golden age of intracellular delivery, in which quite a few physical permeabilization methods were invented. [49][50][51][52][53][54][55][56][57][58] Sonoporation enhances the intracellular delivery of cargos into cells by employing acoustic waves to disrupt their membranes. [22,[59][60][61][62] Traditional sonoporation approaches typically rely on ultrasound-induced bubble dynamic behaviors, such as inertial cavitation, noninertial (stable) cavitation, and bubble translation. [49][50][51][52] However, the bubble-based sonoporation approaches usually require special contrast agents, [63][64][65][66][67][68] which adds a new dimension of complexity and cost to the use of sonoporation. Moreover, bubble-based approaches have a high chance of inducing irreversible cell damage, lowering the cell viability, as well as reducing the effectiveness of delivered cargos. [69][70][71][72][73] Therefore, several novel non-bubble-based sonoporation approaches, such as those based on acoustic radiation force and acoustic streaming-induced shear force, are being developed to overcome the limitations of bubble-based sonoporation approaches. Although there are several review papers on the mechanisms and applications of bubble-based sonoporation, [61,62,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88] they have neglected some of the newly developed mechanisms. Therefore, this review article covers recent innovations in bubble-based and especially in non-bubble-based sonoporation. The following sections review the sonoporation mechanisms, A surge of research in intracellular delivery technologies is underway with the increased innovations in cell-based therapies and cell reprogramming. Particularly, physical cell membrane p...

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Section: Non-bubble-based Mechanismsmentioning

confidence: 99%

Section: Non-bubble-based Mechanismsmentioning

confidence: 99%

Section: Hyper-frequency Bulk Acoustic Wave-based Streaming Technologiesmentioning

confidence: 99%

Section: Hyper-frequency Bulk Acoustic Wave-based Streaming Technologiesmentioning

confidence: 99%

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Sonoporation: Past, Present, and Future

Rich

Tian

Huang

2021

Adv Materials Technologies

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“…[70] With the hydrodynamic force generated from acoustic streaming under gigahertz (GHz) resonator excitation, Guo realized controllable and restorable cell deformation. [71] This resonator can generate localized and high-speed fluid motion because of the rapid attenuation of acoustic energy in liquid environment. Repeating cell extrusion and recovery process can be obtained by applying periodically acoustic streaming with tunable duration.…”

Section: Cell Membrane Deformationmentioning

confidence: 99%

Recent Progress and Perspectives on Cell Surface Modification

Zhang

Zhou

Qian

2021

Chemistry An Asian Journal

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The cell membrane is a biological interface consisting of phospholipid bilayer, saccharides and proteins that maintains a stable metabolic intracellular environment as well as regulating and controlling the exchange of substances inside and outside the cell. Cell membranes provide a highly complex biological surface carrying a variety of essential surfaces ligands and receptors for cells to receive various stimuli of external signals, thereby inducing corresponding cell responses regulating the life activities of the cell. These surface receptors can be manipulated via cell surface modification to regulate cellular functions and behaviors Thus, cell surface modification has attracted considerable attention due to its significance in cell fate control, cell engineering and cell therapy. In this minireview, we describe the recent developments and advances of cell surface modification, and summarize the main modification methods with corresponding functions and applications. Finally, the prospect for the future development of the modification of the living cell membrane is discussed.

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Narrowband Monolithic Perovskite–Perovskite Tandem Photodetectors

Martínez‐Goyeneche

Gil‐Escrig

Susic

et al. 2022

Advanced Optical Materials

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Narrowband photodetectors (PDs) are sought after for many applications requiring selective spectral response. The most common systems combine optical bandpass filters with broadband photodiodes. This work reports a method to obtain a narrowband response in a perovskite PD by the monolithic integration of a perovskite photoconductor and a perovskite photodiode. The spectral response of the tandem PD is determined by the bandgap energy difference of the two perovskites, and exhibits a full width at half maximum below 85 nm, an external quantum efficiency up to 68% and a high specific detectivity of ≈1012 Jones in reverse bias, enabling the device to detect weak light signals. The absorption profile of the narrowband PD can be tuned by changing the thickness and bandgap of the wide bandgap perovskite absorber.

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Controllable Cell Deformation Using Acoustic Streaming for Membrane Permeability Modulation

Cited by 45 publications

References 49 publications

Sonoporation: Past, Present, and Future

Sonoporation: Past, Present, and Future

Recent Progress and Perspectives on Cell Surface Modification

Narrowband Monolithic Perovskite–Perovskite Tandem Photodetectors

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