Control of the Morphology of Lipid Layers by Substrate Surface Chemistry

Abstract

In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. The influence of the properties of the surface coating on formation of the deposited lipid layer was studied with quartz crystal microbalance and two-wavelength multiparametric surface plasmon resonance techniques. Control of lipid self-assembly on the surface was achieved by two different types of soft substrate materials, i.e., dextran and thiolated polyethylene glycol, functionalized with hydrophobic linkers for capturing the lipid layer. The low-molecular-weight dextran-based surface promoted formation of supported lipid bilayers, while the thiolated polyethylene glycol-based surface promoted supported vesicular layer formation. A silicon dioxide surface was used as a reference surface in both measurement techniques. In addition to promoting supported lipid bilayer formation of known lipid mixtures, the dextran surface also promoted supported lipid bilayer formation of vesicles containing the cell membrane extract of human hepatoblastoma cells. The new dextran-based surface was also capable of protecting the supported lipid bilayer against dehydration when exposed to a constant flow of air. The well-established quartz crystal microbalance technique was effective in determining the morphology of the formed lipid layer, while the two-wavelength surface plasmon resonance analysis enabled further complementary characterization of the adsorbed supported lipid bilayers and supported vesicular layers.

Detection of Phase Transition in Photosensitive Liposomes by Advanced QCM

“…al. 25 . After 24 h, the crystals were washed with ethanol and water and dried with a stream of nitrogen.…”

Preserved Transmembrane Protein Mobility in Polymer-Supported Lipid Bilayers Derived from Cell Membranes

“…Several strategies to induce vesicle-substrate fusion of such systems have been investigated 19 , including α-helical (AH) peptide-induced fusion 14 , bilayer edge-induced fusion 16,17 , and synthetic/native membrane vesicle co-adsorption 11,12 . Yet, in the few reports where native membrane vesicles have been used to produce SLBs the mobility of membrane proteins within the SLBs has been either very poor or non-disclosed [11][12][13]20 . The loss of membrane protein mobility is generally attributed to irreversible adsorption of proteins to the substrate which is most likely to occur at the critical stage of vesicle adsorption, at which point transmembrane proteins with protruding domains can be in direct contact with the substrate.…”

Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

“…Previous SPR studies on the interactions of nanocarriers with blood proteins have employed diluted plasma [13,23,25]. The surface of biosensors can be functionalized with specific chemistries of interest, proteins, or a hydrogel and lipid matrix that can be used to capture nanocarriers on the sensor surfacean approach we utilize in this work among others [14,[25][26][27].…”