Control of superoxide and nitric oxide formation during human sperm capacitation

Lamirande, Eve de; Lamothe, Geneviève; Villemure, Michèle

doi:10.1016/j.freeradbiomed.2009.02.022

Cited by 54 publications

(54 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surprisingly, the absence of intracellular Ca 2þ in boar spermatozoa by incubation with 1,2-bis(2-aminophenoxy)ethane-N,N,N 0 ,N 0 -tetraacetic acid/acetoxy methyl (BAPTA-AM) in a Ca 2þ -free media leads to a strong increase in AMPK activity [51], which may be mediated through an increase in nitric oxide NO$ production. In this sense, de Lamirande et al [69] reported in human sperm that BAPTA-AM promotes the production of an ROS, the nitric oxide NO$. Accordingly, AMPK activation is also directly influenced by cellular redox status in somatic cells, as H 2 O 2 activates AMPK through oxidative modification of cysteine residues in the AMPKa subunit [70].…”

Section: Signaling Pathways Underlying Ampk Activity In Boar Spermatozoamentioning

confidence: 99%

New insights into transduction pathways that regulate boar sperm function

et al. 2016

View full text Add to dashboard Cite

Section: Signaling Pathways Underlying Ampk Activity In Boar Spermatozoamentioning

confidence: 99%

New insights into transduction pathways that regulate boar sperm function

et al. 2016

View full text Add to dashboard Cite

“…-play decisive roles in regulating multiple functions within the male reproductive system either separately or through mutual interactions (1).…”

Section: No and Omentioning

confidence: 99%

“…To examine the role of O 2 -and NO in reproduction, the use of compounds that modulate the activity of enzymes involved in their generation/removal have proved useful (1). However, the use of native enzymes in clinical trials is limited, and synthetic enzyme mimics have emerged as promising candidates.…”

mentioning

confidence: 99%

Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?

Otašević

Korać

Vučetić

et al. 2013

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ( NO), the number of MitoTracker Ò Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as NO might be involved in the observed effects of the mimic.

show abstract

“…Different oxygen radicals such as superoxide anion, nitric oxide, and hydrogen peroxide have been related to sperm capacitation (Griveau et al 1994, de Lamirande et al 2009) and the addition of ROS yields an increase in PY in a variety of species (Baumber et al 2003, Roy & Atreja 2008, Kota et al 2010, Dona et al 2011. It has recently been shown that ROS generation during capacitation may be necessary for cholesterol efflux from the sperm plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Macías‐García¹,

González‐Fernández²,

Loux³

et al. 2015

Reproduction

View full text Add to dashboard Cite

Repeatable methods for IVF have not been established in the horse, reflecting the failure of standard capacitating media to induce changes required for fertilization capacity in equine sperm. One important step in capacitation is membrane cholesterol efflux, which in other species is triggered by cholesterol oxidation and is typically enhanced using albumin as a sterol acceptor. We incubated equine sperm in the presence of calcium, BSA, and bicarbonate, alone or in combination. Bicarbonate induced an increase in reactive oxygen species (ROS) that was abolished by the addition of calcium or BSA. Bicarbonate induced protein tyrosine phosphorylation (PY), even in the presence of calcium or BSA. Incubation at high pH enhanced PY but did not increase ROS production. Notably, no combination of these factors was associated with significant cholesterol efflux, as assessed by fluorescent quantitative cholesterol assay and confirmed by filipin staining. By contrast, sperm treated with methyl-b-cyclodextrin showed a significant reduction in cholesterol levels, but no significant increase in PY or ROS. Presence of BSA increased sperm binding to bovine zonae pellucidae in all three stallions. These results show that presence of serum albumin is not associated with a reduction in membrane cholesterol levels in equine sperm, highlighting the failure of equine sperm to exhibit core capacitation-related changes in a standard capacitating medium. These data indicate an atypical relationship among cholesterol efflux, ROS production, and PY in equine sperm. Our findings may help to elucidate factors affecting failure of equine IVF under standard conditions.

show abstract

Control of superoxide and nitric oxide formation during human sperm capacitation

Cited by 54 publications

References 54 publications

New insights into transduction pathways that regulate boar sperm function

New insights into transduction pathways that regulate boar sperm function

Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Contact Info

Product

Resources

About