Control of gene expression by base deamination: The case of RNA editing in wheat mitochondria

Wheat Mitochondrial Proteomes Provide New Links between Antioxidant Defense and Plant Salinity Tolerance

“…Mitochondria have been isolated from wheat and utilized in foundational plant biology studies for over four decades, investigating plant respiratory metabolism, cytoplasmic male sterility, the mitochondrial genome, organelle RNA processing and editing and the composition of electron transport chain complexes . However, a systematic analysis of the wheat mitochondrial proteome has not been reported.…”

Wheat Mitochondrial Proteomes Provide New Links between Antioxidant Defense and Plant Salinity Tolerance

“…Mitochondria have been isolated from wheat and utilized in foundational plant biology studies for over four decades, investigating plant respiratory metabolism, cytoplasmic male sterility, the mitochondrial genome, organelle RNA processing and editing and the composition of electron transport chain complexes . However, a systematic analysis of the wheat mitochondrial proteome has not been reported.…”

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding

“…Unfortunately, they were not suitable to study the enzymatic mechanism involved in this reaction. Thus, we settled a simplified in vitro system for routine assays which uses an in vitro radioactively labelled atp9 mRNA as substrate (Blanc et al, 1995;Blanc et al, 1996). After incubation with the mitochondrial lysate the edited product was extensively digested with nuclease P1 and submitted to a double dimension thin-layer chromatography (TLC) to separate the 5' NMPs.…”

Gene expression studies in isolated mitochondria: Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription, splicing and editing machinery of wheat mitochondria

“…RNA editing has been found in a variety of organisms and occurs through different mechanisms such as insertion or deletion of nucleotides, or base conversions [for details see reference ( 2 )]. In higher plant organelles, RNA editing is an important post-transcriptional event characterized by C-to-U changes via a deamination mechanism ( 3 – 5 ). In Arabidopsis thaliana 456 C-to-U editing events have been described ( 6 ).…”

Wheat Mitochondrial Proteomes Provide New Links between Antioxidant Defense and Plant Salinity Tolerance

“…Mitochondria have been isolated from wheat and utilized in foundational plant biology studies for over four decades, investigating plant respiratory metabolism, cytoplasmic male sterility, the mitochondrial genome, organelle RNA processing and editing and the composition of electron transport chain complexes . However, a systematic analysis of the wheat mitochondrial proteome has not been reported.…”

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding

“…Unfortunately, they were not suitable to study the enzymatic mechanism involved in this reaction. Thus, we settled a simplified in vitro system for routine assays which uses an in vitro radioactively labelled atp9 mRNA as substrate (Blanc et al, 1995;Blanc et al, 1996). After incubation with the mitochondrial lysate the edited product was extensively digested with nuclease P1 and submitted to a double dimension thin-layer chromatography (TLC) to separate the 5' NMPs.…”

Gene expression studies in isolated mitochondria: Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription, splicing and editing machinery of wheat mitochondria

“…RNA editing has been found in a variety of organisms and occurs through different mechanisms such as insertion or deletion of nucleotides, or base conversions [for details see reference ( 2 )]. In higher plant organelles, RNA editing is an important post-transcriptional event characterized by C-to-U changes via a deamination mechanism ( 3 – 5 ). In Arabidopsis thaliana 456 C-to-U editing events have been described ( 6 ).…”

Wheat Mitochondrial Proteomes Provide New Links between Antioxidant Defense and Plant Salinity Tolerance

“…Mitochondria have been isolated from wheat and utilized in foundational plant biology studies for over four decades, investigating plant respiratory metabolism, cytoplasmic male sterility, the mitochondrial genome, organelle RNA processing and editing and the composition of electron transport chain complexes . However, a systematic analysis of the wheat mitochondrial proteome has not been reported.…”

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding

“…Unfortunately, they were not suitable to study the enzymatic mechanism involved in this reaction. Thus, we settled a simplified in vitro system for routine assays which uses an in vitro radioactively labelled atp9 mRNA as substrate (Blanc et al, 1995;Blanc et al, 1996). After incubation with the mitochondrial lysate the edited product was extensively digested with nuclease P1 and submitted to a double dimension thin-layer chromatography (TLC) to separate the 5' NMPs.…”

Gene expression studies in isolated mitochondria: Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription, splicing and editing machinery of wheat mitochondria

“…RNA editing has been found in a variety of organisms and occurs through different mechanisms such as insertion or deletion of nucleotides, or base conversions [for details see reference ( 2 )]. In higher plant organelles, RNA editing is an important post-transcriptional event characterized by C-to-U changes via a deamination mechanism ( 3 – 5 ). In Arabidopsis thaliana 456 C-to-U editing events have been described ( 6 ).…”