2006
DOI: 10.1111/j.1567-1364.2006.00038.x
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Abstract: The goal of this study was to develop conditional flocculant yeasts for use in the alcohol fermentation industry. Promoters were constructed to completely repress gene transcription in the presence of sugar and to support strong expression after the exhaustion of this compound. A fusion of regulatory regions of the ADH2 promoter with the FLO5 core promoter was constructed to regulate the FLO5 gene. This construct was inserted into multicopy plasmids and transformed into laboratory strains of Saccharomyces cere… Show more

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Cited by 30 publications
(24 citation statements)
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“…In contrast, several studies 10,16,20,102,109 show that the timing and strength of flocculation can be successfully controlled in a planned way by genetic engineering, although it may sometimes be difficult to find the tools (promoters etc.) giving the required behaviour (see, e.g.…”
Section: Discussionmentioning
confidence: 99%
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“…In contrast, several studies 10,16,20,102,109 show that the timing and strength of flocculation can be successfully controlled in a planned way by genetic engineering, although it may sometimes be difficult to find the tools (promoters etc.) giving the required behaviour (see, e.g.…”
Section: Discussionmentioning
confidence: 99%
“…giving the required behaviour (see, e.g. 10 ). In some cases, genetic engineering of flocculation characteristics appeared to alter other fermentation characteristics in ways not completely understood.…”
Section: Discussionmentioning
confidence: 99%
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“…The distinct advantage of the cloning strategy employed here over those used by other research groups (10,12) is that no subcloning of the FLO genes is required. Furthermore, expression levels are independent of plasmid-related artifacts, such as variable copy numbers and the increased risk of intragenic recombinations.…”
Section: Discussionmentioning
confidence: 99%
“…6,7) The mechanism of yeast flocculation involves the interaction of lectin-like proteins, called flocculins, with receptors on a neighboring cell wall, resulting in the formation of aggregates. [8][9][10][11] Environmental factors such as pH, calcium, and organic stress also affect flocculation. 9,[12][13][14] The five FLO genes, FLO1, FLO5, FLO9, FLO10, and FLO11, have been described in S. cerevisiae thus far.…”
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confidence: 99%