“…PCRs were performed with primer pairs listed in Table 1 in a mycircle PCR system (Bio-Rad) under conditions described previously (Everett, Jin, Ricci, & Piddock, 1996). Briefly, PCR was carried out in a 25 μL PCR mixture which contained 0.5 μM of each primer, 250 μM of each dNTP, 1 × PCR buffer, 1.5 mM MgCl 2 , 0.5 U of Ex Taq DNA polymerase (TaKaRa) and 5 μL of sample DNA with incubation at 94°C for 10 min, followed by 35 cycles of 94°C for 30 s, 56°C for 30 s and 72°C for 30 s, and a final extension of 72°C for 7 min.…”