1996
DOI: 10.1128/aac.40.10.2380
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Abstract: Twenty-eight human isolates of Escherichia coli from Argentina and Spain and eight veterinary isolates received from the Ministry of Agriculture Fisheries and Foods in the United Kingdom required 2 to > 128 micrograms of ciprofloxacin per ml for inhibition. Fragments of gyrA and parC encompassing the quinolone resistance-determining region were amplified by PCR, and the DNA sequences of the fragments were determined. All isolates contained a mutation in gyrA of a serine at position 83 (Ser83) to an Leu, and… Show more

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Cited by 326 publications
(195 citation statements)
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“…The exact role of amino acid substitutions in parE is unclear. Everett et al (1996) and Ruiz et al (1997) reported that the development of fluoroquinolone resistance in clinical isolates of Gram-negative bacteria appeared to be irrelevant to parE mutation. No gyrB mutations were identified in the present study, although gyrB mutations have been reported previously in Salmonella and E. coli (Eaves et al, 2004;Giraud et al, 2006).…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…The exact role of amino acid substitutions in parE is unclear. Everett et al (1996) and Ruiz et al (1997) reported that the development of fluoroquinolone resistance in clinical isolates of Gram-negative bacteria appeared to be irrelevant to parE mutation. No gyrB mutations were identified in the present study, although gyrB mutations have been reported previously in Salmonella and E. coli (Eaves et al, 2004;Giraud et al, 2006).…”
Section: Discussionmentioning
confidence: 97%
“…PCRs were performed with primer pairs listed in Table 1 in a mycircle PCR system (Bio-Rad) under conditions described previously (Everett, Jin, Ricci, & Piddock, 1996). Briefly, PCR was carried out in a 25 μL PCR mixture which contained 0.5 μM of each primer, 250 μM of each dNTP, 1 × PCR buffer, 1.5 mM MgCl 2 , 0.5 U of Ex Taq DNA polymerase (TaKaRa) and 5 μL of sample DNA with incubation at 94°C for 10 min, followed by 35 cycles of 94°C for 30 s, 56°C for 30 s and 72°C for 30 s, and a final extension of 72°C for 7 min.…”
Section: Amplification and Sequencing Of Gyra Gyrb Parc And Pare Genesmentioning
confidence: 99%
“…E. coli isolates with low level of MIC for CIP (0.1-0.5 mg/L) and ENR (1-4 mg/L) represented a single mutation; intermediary MIC for CIP (1-2 mg/L) and ENR (4-8 mg/L) were related with two mutations (one gyrA and one parC) and high-level resistance MIC for CIP ( ≥ 4 mg/L) and ENR ( ≥ 16 mg/L) represented three mutations (two in gyrA and one in parC). For the detection of resistance-mediating mutations in the target genes, QRDR of gyrA and parC were amplified and sequenced (Everett et al 1996).…”
Section: Methodsmentioning
confidence: 99%
“…Some isolates with a lack of or lower expression of porins, such as OmpF and OmpC, have been described [10,29]. Chenia et al [10] studied the accumulation of ciprofloxacin in six E. coli clinical isolates (five isolates had a ciprofloxacin MIC of 32 g/mL) and found that after adding 100 M of carbonyl cyanide mchlorophenylhydrazone (CCCP), an electron transport chain uncoupler that inhibits energy-dependent efflux, the internal accumulation significantly rose, suggesting that an efflux mechanism is present and active in these isolates.…”
Section: Mechanisms Of Resistance To Quinolones In Escherichia Colimentioning
confidence: 99%