Contrasting central and systemic effects of arginine-vasopressin on urinary marking behavior as a social signal in male mice

Higuchi, Yuki; Arakawa, Hiroyuki

doi:10.1016/j.yhbeh.2022.105128

Cited by 5 publications

(2 citation statements)

References 79 publications

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“…Testing was performed in tandem with small groups of mice (three-to-four) at different times. The sample size of 10-12 per group was determined by a power calculation via a data set from previous experiments (Higuchi & Arakawa, 2022).…”

Section: Experimental Designmentioning

confidence: 99%

The role of the posterior intralaminar thalamic nucleus on contact sequence in mouse social interaction

Arakawa,

Tokashiki

2024

Preprint

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Efficient social interaction is essential for an adaptive life and consists of sequential processes of multisensory events with social counterparts. Social touch/contact is a unique component that promotes a sequence of social behaviors initiated by detection and approach to assess a social stimulus and subsequent maintenance of touch/contacts to form prosocial relationships. We hypothesized that the thalamic sensory relay circuit from the posterior intralaminar nucleus of the thalamus (pIL) to the paraventricular nucleus of the hypothalamus (PVN) and the medial amygdala (MeA) plays a key role in the social contact-mediated sequence of events. We found that neurons in the pIL along with the PVN and MeA, were activated by social encounters, and that pIL activity was more abundant in a direct physical encounter, whereas MeA activity was dominant in an indirect via grid encounter. Chemogenetic inhibition of pIL neurons selectively decreased the investigatory approach and sniffing of a same-sex, but not an opposite-sex, stimulus mouse in an indirect encounter situation and decreased the facial/snout contact ratio in a direct encounter setting. Furthermore, chemogenetic pIL inhibition had no impact on anxiety-like behaviors or body coordinative motor behaviors, but it impaired whisker-related and plantar touch tactile sense. We propose that the pIL circuit can relay social tactile sensations and mediate the sequence of non-sexual prosocial interactions using an investigatory approach through tactile contact.

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Section: Experimental Designmentioning

confidence: 99%

The role of the posterior intralaminar thalamic nucleus on contact sequence in mouse social interaction

Arakawa,

Tokashiki

2024

Preprint

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“…Urination pattern is traditionally analyzed by the void spot assay, which uses filter paper placed on the arena’s floor for analyzing urine deposit distribution after the end of the experiment Wolff and Powell (1984); Higuchi and Arakawa (2022). However, this analysis usually lacks the time dimension, is affected by urine smearing and spreading across the arena floor due to the mouse movement (see Figure 2 d,e), and is limited in detecting overlapping urine spots.…”

Section: Introductionmentioning

confidence: 99%

DeePosit: an AI-based tool for detecting mouse urine and fecal depositions from thermal video clips of behavioral experiments

Peles,

Netser,

Ray

et al. 2024

Preprint

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In many mammals, including rodents, social interaction is accompanied by active urination, also known as micturition, for spatial scent marking. Urine and fecal deposits were shown to contain multiple chemosensory cues carrying information regarding the identity, strain, and social rank, as well as the physiological and hormonal conditions of the individual. Moreover, scent marking was shown to be social context-, state-, and experience-dependent. Thus, analyzing scent-marking activity during social interactions may contribute to understanding the structure of mammalian social interactions in health and disease. So far, however, such analysis faced multiple technical obstacles. Mainly, the commonly used void spot assay relies on detecting urine traces left over a filter paper on which the social interaction occurred; thus, it lacks temporal information and is prone to artifacts such as urine smearing. Recently, several studies employed thermal imaging for spatiotemporal analysis of scent marking, as urine and feces are deposited at body temperature and get rapidly cold afterward. This analysis, however, was done so far manually, which made it time-consuming and prone to bias by the observer. Here, we combine thermal imaging, computer vision tools, and an open-source algorithm incorporating a transformer-based video classifier to automatically detect and classify urine and fecal deposits made by male and female mice during several social behavior tests. We found distinct dynamics for urine and fecal depositions in a test- and sex-dependent manner, suggesting two distinct processes of scent marking in mice. The method and tools presented here allow researchers an easy, efficient, and unbiased spatiotemporal analysis of scent marking during behavioral experiments.

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The Usability of Mouse Models to Study the Neural Circuity in Autism Spectrum Disorder: Regulatory Mechanisms of Core Behavioral Symptoms

Arakawa,

Higuchi

2023

Neurobiology of Autism Spectrum Disorders

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Contrasting central and systemic effects of arginine-vasopressin on urinary marking behavior as a social signal in male mice

Cited by 5 publications

References 79 publications

The role of the posterior intralaminar thalamic nucleus on contact sequence in mouse social interaction

The role of the posterior intralaminar thalamic nucleus on contact sequence in mouse social interaction

DeePosit: an AI-based tool for detecting mouse urine and fecal depositions from thermal video clips of behavioral experiments

The Usability of Mouse Models to Study the Neural Circuity in Autism Spectrum Disorder: Regulatory Mechanisms of Core Behavioral Symptoms

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