Contrast Agents for Optical Imaging

Licha, Kai

doi:10.1007/3-540-46009-8_1

Cited by 156 publications

(92 citation statements)

References 138 publications

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“…One of the most important considerations in optical imaging is maximizing the depth of tissue penetration. Absorption and scattering of light are largely a function of the wavelength of the excitation source [3]. Light is absorbed by endogenous chromophores found in living tissue, including hemoglobin, melanin, and lipid [3], [4], [5], [6], and [7].…”

Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

“…In general, light absorption and scattering decrease with increasing wavelength. Below ~700 nm, these effects result in shallow penetration depths of only a few millimeters [3]. Thus, in the visible region of the spectrum, only superfi cial assessment of tissue features is possible.…”

Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

“…Above 900 nm, water absorption can interfere with signal-to-background ratio. Because the absorption coeffi cient of tissue is considerably lower in the near infrared (NIR) region (700-900 nm), light can penetrate more deeply, to depths of several centimeters [3], [4], [5] and [6]. Fluorochromes with emissions in the NIR are not hindered by interfering autofl uorescence, so they tend to yield the highest signal-to-background.…”

Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

“…Important criteria for effective optical imaging fl uorochromes include excitation and emission maxima in the NIR between 700 and 900 nm, high quantum yield [3], [4], [5], [6] and [7], chemical and optical stability, and suitable pharmacological properties including aqueous solubility, low nonspecifi c binding, rapid clearance of the free dye, and low toxicity. The dyes most commonly used for fl uorescent optical imaging are listed in Table 1.…”

Section: Near Infrared Fl Uorochromesmentioning

confidence: 99%

See 3 more Smart Citations

A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

Kovar

Simpson

Schutz-Geschwender

et al. 2007

Analytical Biochemistry

141

142

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Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

Section: Principles Of Fl Uorescence Imagingmentioning

confidence: 99%

Section: Near Infrared Fl Uorochromesmentioning

confidence: 99%

See 2 more Smart Citations

A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

Kovar

Simpson

Schutz-Geschwender

et al. 2007

Analytical Biochemistry

141

142

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“…Below ~700 nm the wavelength of the fluorescent dye is too low to be detected further than a few hundreds of micrometers of tissue penetration. 2 Between 700 nm and 900 nm (the near-infrared region, NIR) absorption is at its lowest. Usage of dyes with wavelengths above 900 nm is not possible because water absorbs very strongly at these wavelengths, dramatically reducing the efficacy of light transmission.…”

Section: Fluorescent Dye Characteristicsmentioning

confidence: 99%

The use of fluorescent dyes and probes in surgical oncology

Velde

Veerman

Subramaniam

et al. 2010

European Journal of Surgical Oncology (EJSO)

130

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Aims and background: Improved visualization of surgical targets inside of the patient helps to improve radical resection of the tumor while sparing healthy surrounding tissue. In order to achieve an image, optical contrast must be generated by properties intrinsic to the tissue, or require the attachment of special visualization labels to the tumor. In this overview the current status of the clinical use of fluorescent dyes and probes are reviewed. Methods:In this review, all experimental and clinical studies concerning fluorescent imaging were included. In addition, in the search for the optimal fluorescent imaging modality, all characteristics of a fluorescent dye were described. Findings and Conclusions:Although the technique of imaging through fluorescence sounds promising and several animal models show efficacy, official approval of these agents for further clinical evaluation, is eagerly awaited.

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Near‐Infrared Molecular Probes for In Vivo Imaging

et al. 2012

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Cellular and tissue imaging in the near‐infrared (NIR) wavelengths between 700 and 900 nm is advantageous for in vivo imaging because of the low absorption of biological molecules in this region. This unit presents protocols for small animal imaging using planar and fluorescence lifetime imaging techniques. Included is an overview of NIR fluorescence imaging of cells and small animals using NIR organic fluorophores, nanoparticles, and multimodal imaging probes. The development, advantages, and application of NIR fluorescent probes that have been used for in vivo imaging are also summarized. The use of NIR agents in conjunction with visible dyes and considerations in selecting imaging agents are discussed. We conclude with practical considerations for the use of these dyes in cell and small animal imaging applications. Curr. Protoc. Cytom. 60:12.27.1‐12.27.20. © 2012 by John Wiley & Sons, Inc.

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Contrast Agents for Optical Imaging

Cited by 156 publications

References 138 publications

A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

The use of fluorescent dyes and probes in surgical oncology

Near‐Infrared Molecular Probes for In Vivo Imaging

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