Contraction in Stentor coeruleus induced by electric stimulation

Temperature effects upon the time course of contraction and reextension in Stentor coeruleus

“…Stentor coeruleus was cultured as previously described and the experimental set-up was essentially similar to the one described in a previous work (Nielsen, 1971). The animals were transferred into the test chamber, which was 2 mm high, 2 x 3 cm wide and long, with top and bottom made of glass.…”

“…The temperature in the test chamber was measured by a cobalttcopper thermocouple, using an ice bath for reference and having the voltage read in a digital voltmeter (Newport 2003A). The time course of contraction was followed by photographing the animals by the double flash technique previously described (Nielsen, 1971). Hereby two electronic light flashes from an electronic flash lamp beneath the test chamber resulted in two superpositioned photomicrographs of the same Stentor on one frame.…”

Temperature effects upon the time course of contraction and reextension in Stentor coeruleus

“…Stentor coeruleus was cultured as previously described and the experimental set-up was essentially similar to the one described in a previous work (Nielsen, 1971). The animals were transferred into the test chamber, which was 2 mm high, 2 x 3 cm wide and long, with top and bottom made of glass.…”

“…The temperature in the test chamber was measured by a cobalttcopper thermocouple, using an ice bath for reference and having the voltage read in a digital voltmeter (Newport 2003A). The time course of contraction was followed by photographing the animals by the double flash technique previously described (Nielsen, 1971). Hereby two electronic light flashes from an electronic flash lamp beneath the test chamber resulted in two superpositioned photomicrographs of the same Stentor on one frame.…”

Temperature effects upon the time course of contraction and reextension in Stentor coeruleus

“…Stentor coeruleus was cultured as previously described and the experimental set-up was essentially similar to the one described in a previous work (Nielsen, 1971). The animals were transferred into the test chamber, which was 2 mm high, 2 x 3 cm wide and long, with top and bottom made of glass.…”

“…The temperature in the test chamber was measured by a cobalttcopper thermocouple, using an ice bath for reference and having the voltage read in a digital voltmeter (Newport 2003A). The time course of contraction was followed by photographing the animals by the double flash technique previously described (Nielsen, 1971). Hereby two electronic light flashes from an electronic flash lamp beneath the test chamber resulted in two superpositioned photomicrographs of the same Stentor on one frame.…”

Temperature effects upon the time course of contraction and reextension in Stentor coeruleus

“…Stentor coeruleus was cultured as previously described and the experimental set-up was essentially similar to the one described in a previous work (Nielsen, 1971). The animals were transferred into the test chamber, which was 2 mm high, 2 x 3 cm wide and long, with top and bottom made of glass.…”

“…The temperature in the test chamber was measured by a cobalttcopper thermocouple, using an ice bath for reference and having the voltage read in a digital voltmeter (Newport 2003A). The time course of contraction was followed by photographing the animals by the double flash technique previously described (Nielsen, 1971). Hereby two electronic light flashes from an electronic flash lamp beneath the test chamber resulted in two superpositioned photomicrographs of the same Stentor on one frame.…”