Animal Cell Culture and Production of Biologicals 1991
DOI: 10.1007/978-94-011-3550-4_38
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Cited by 4 publications
(3 citation statements)
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“…Cell densities of 1.1 X 108 cells/ml-matrix were achieved with total cell number exceeding 4.4 X 101° cells/400 ml-bioreactoL In the previous study, this system proved that production of erythropoietin reached 1.0 × 106 arbitrary units/day/400 ml-bioreactor by using recombinant Chinese hamster's ovary (CHO) ceils, and that of interleukin-6 (IL-6) reached 8.3 arbitrary units/day/400 ml-bioreactor by using recombinant hamster's kidney (BHK) cells (Mizutani, 1990(Mizutani, , 1992Yoshida et al, 1991Yoshida et al, , 1993. Cell densities of 1.1 X 108 cells/ml-matrix were achieved with total cell number exceeding 4.4 X 101° cells/400 ml-bioreactoL In the previous study, this system proved that production of erythropoietin reached 1.0 × 106 arbitrary units/day/400 ml-bioreactor by using recombinant Chinese hamster's ovary (CHO) ceils, and that of interleukin-6 (IL-6) reached 8.3 arbitrary units/day/400 ml-bioreactor by using recombinant hamster's kidney (BHK) cells (Mizutani, 1990(Mizutani, , 1992Yoshida et al, 1991Yoshida et al, , 1993.…”
mentioning
confidence: 89%
“…Cell densities of 1.1 X 108 cells/ml-matrix were achieved with total cell number exceeding 4.4 X 101° cells/400 ml-bioreactoL In the previous study, this system proved that production of erythropoietin reached 1.0 × 106 arbitrary units/day/400 ml-bioreactor by using recombinant Chinese hamster's ovary (CHO) ceils, and that of interleukin-6 (IL-6) reached 8.3 arbitrary units/day/400 ml-bioreactor by using recombinant hamster's kidney (BHK) cells (Mizutani, 1990(Mizutani, , 1992Yoshida et al, 1991Yoshida et al, , 1993. Cell densities of 1.1 X 108 cells/ml-matrix were achieved with total cell number exceeding 4.4 X 101° cells/400 ml-bioreactoL In the previous study, this system proved that production of erythropoietin reached 1.0 × 106 arbitrary units/day/400 ml-bioreactor by using recombinant Chinese hamster's ovary (CHO) ceils, and that of interleukin-6 (IL-6) reached 8.3 arbitrary units/day/400 ml-bioreactor by using recombinant hamster's kidney (BHK) cells (Mizutani, 1990(Mizutani, , 1992Yoshida et al, 1991Yoshida et al, , 1993.…”
mentioning
confidence: 89%
“…2. [48][49][50][51] The RFB column consists of a vertically extended cylindrical matrix comprised of porous glass bead microcarriers, through which liquid medium flows from the periphery toward the central axis, generating a beneficial concentration gradient of oxygen and nutrients, while preventing excessive shear Fig. 2.…”
Section: The Three-dimensional Bioreactormentioning
confidence: 99%
“…23 A further research study has demonstrated that the system supports highdensity, large-scale cell cultures having a long-term capacity to maintain multiple liver-specific functions, such as protein synthesis, enzyme activity, and drug metabolism, both quantitatively and qualitatively. 53 In summary, the RFB system has overcome the following problems associated with conventional BAL reactors: [49][50][51]54 (1) Influence of shear stress (2) Uneven supply of oxygen and nutrients (3) Build-up of waste substances (4) Gravitational sedimentation of cells (5) Incomplete three-dimensional culture (6) Poor cost-to-performance ratio (7) Difficulty to scale up or scale down. Employing this RFB system together with the FLC cell lines 55 developed in our laboratory, we have confirmed rapid and prolonged proliferation of FLC cell cultures showing normal levels of oxygen and glucose consumption, as well as albumin synthesis and ammonia metabolism.…”
Section: The Three-dimensional Bioreactormentioning
confidence: 99%