Continuous intrapulmonary distension with perfluorocarbon accelerates neonatal (but not adult) lung growth

Nobuhara, Kerilyn K.; Fauza, Dario O.; DiFiore, John W.; Hines, Michael H.; Fackler, James C.; Slavin, Richard E.; Hirschl, Ronald B.; Wilson, Jay M.

doi:10.1016/s0022-3468(98)90450-x

Cited by 72 publications

(39 citation statements)

References 29 publications

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“…In diaphragmatic hernia the abdominal organs are displaced into the thoracic cavity, thereby precluding normal lung expansion and indirectly decreasing the airway hydrostatic pressure (26,27). Diminished intrapulmonary pressure and impaired distention result in less mechanical stretch (24)(25)(26)(27)(28)(29)(30), inasmuch as correcting the latter in utero by artificially increasing the airway hydrostatic pressure ameliorates these two types of lung hypoplasia (26,29,30).…”

Section: Discussionmentioning

confidence: 99%

Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia

Yang¹,

Beqaj²,

Kemp³

et al. 2000

J. Clin. Invest.

111

101

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IntroductionVisceral smooth muscle (SM) originates from local mesenchymal cells that in early-midgestation begin to synthesize SM proteins, including SM α-actin, desmin, SM myosin, SM22, and calponin in a specific periairway distribution (1-5). In the mouse developing respiratory system, cells expressing SM proteins are first detected in the trachea on day 11 of gestation (3, 4), and then SM differentiation proceeds in a cranial-tocaudal fashion to form the bronchial musculature (1-5). The other type of visceral SM cells found in the lung are interstitial SM cells, also known as interstitial contractile cells, or myofibroblasts. Interstitial SM cells are originally located at the sites of future alveolar septae, and, in the mature organ, they form part of the septae tips (6). Except for the aorta, the development of the vascular musculature lags behind that of visceral SM by several days (4, 7-9).Unlike striated muscle differentiation, on which considerable information was gathered over the years, the mechanisms and genetic program that control SM myogenesis remain, for the most part, unknown. We and others have observed that lung mesenchymal cell precursors change their shape from round to elongated before undergoing bronchial SM differentiation (ref.3; Y. Yang and L. Schuger, unpublished observations). Based on this observation we recently examined whether changes in cell shape might play a role in airway myogenesis. Unexpectedly, our studies demonstrated that essentially all undifferentiated embryonic mesenchymal cells are potential SM precursors (10-12). These studies also confirmed the critical role of cell shape in myogenesis. Specifically, we found that cell rounding prezvents myogenesis, regardless of the normal fate of the cell in vivo, whereas cell spreading/elongation induces SM differentiation, even in mesenchymal cells from nonmuscular organs (10-12).Developing tubular tissues, such as those of the respiratory, gastrointestinal, and urinary systems, are filled with liquid. As a consequence, the periluminal mesenchymal cells are subjected to mechanical tension/stretch exerted by the liquid's hydrostatic pressure (13). These forces likely represent a significant factor in determining the periluminal mesenchymal cell shape. In the developing lung, cells are additionally subjected to repeated stretch caused by intrauterine breathing (13). The fact that mechanical stretch causes cell elongation and that cell elongation is likely to be sensed by the cell as a mechanical stimulus suggested to us that cell tension/stretch may play an important role in the process of visceral myogenesis.Here we used a combination of lung cell and organ cultures from fetal mouse and human origin to determine the effect of mechanical stretch upon SM myogenesis. Smooth muscle (SM) develops only in organs and sites that sustain mechanical tensions. Therefore, we determined the role of stretch in mouse and human bronchial myogenesis. Sustained stretch induced expression of SM proteins in undifferentiated mesenchymal cells and acc...

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Section: Discussionmentioning

confidence: 99%

Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia

Yang¹,

Beqaj²,

Kemp³

et al. 2000

J. Clin. Invest.

111

101

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“…In contrast, previous studies have shown that the application of CPAP (6 cmH 2 O) for 2 weeks stimulated lung growth in ferret pups (15). Similarly, increased lung expansion for 21 d (but not 7 d) in lambs, using a liquid perfluorocarbon, has been shown to stimulate lung growth (16,31). Combined with the findings of our study, these results suggest that, in comparison with the fetus, the acceleration in lung growth induced by an increase in lung expansion is delayed in postnatal animals.…”

mentioning

confidence: 68%

“…These data suggest that increased lung expansion also regulates lung growth after birth, just as it does in the fetus before birth (1,2). However, studies in fetal sheep (3) indicate that the lung growth response to increased lung expansion is time-dependent and occurs over a much shorter time period (2-3 d) than that previously studied in newborn animals (Ͼ2 wk) (15,16). Thus, our second aim was to determine the effect of short-term periods (12 h) of increased end expiratory lung volumes on pulmonary DNA synthesis rates in newborn lambs.…”

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confidence: 90%

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Effect of Increased Lung Expansion on Surfactant Protein mRNA Levels in Lambs

et al. 2001

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“…In a small series of human fetuses with CDH, where the trachea has been occluded by intrauterine surgery, an accelerated lung growth has been seen [7]. Induced postnatal lung growth has been achieved experimentally by distension of the lungs with perfluorocarbon [8]. This has been used in a small series of human beings with CDH [9].…”

mentioning

confidence: 99%

Insulinlike growth factor I gene expression is increased in the fetal lung after tracheal ligation

Frenckner¹,

Eklöf²,

Eriksson

et al. 2005

Journal of Pediatric Surgery

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Continuous intrapulmonary distension with perfluorocarbon accelerates neonatal (but not adult) lung growth

Cited by 72 publications

References 29 publications

Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia

Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia

Effect of Increased Lung Expansion on Surfactant Protein mRNA Levels in Lambs

Insulinlike growth factor I gene expression is increased in the fetal lung after tracheal ligation

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