Continuous aqueous two-phase systems devices for the recovery of biological products

Bromelain: an overview of industrial application and purification strategies

“…The temperature-induced phase partitioning method will offer cost-effectiveness and recyclability to overcome the bulk industrial waste disposal of polymer. Finally, pre-evaluation and detailed cost comparison with other separation methods must be taken into account to determine the viability of the ATPS process in an industrial scale (Espitia-Saloma et al 2013). …”

Perspectives on integrated continuous bioprocessing—opportunities and challenges

“…These include protein precipitation [5] and crystallization [31], both of which tend to be more selective as the product titer increases, as well as aqueous two-phase extraction [32]. Although precipitation and crystallization are often thought of as batch operations, they can also be performed in a continuous mode using a tubular reactor consisting of a long tube with a static mixer insert to obtain uniform residence time and effective mixing of the reagents and of the formed precipitate [33].…”

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

“…ATPS form when an aqueous solution exceeds a critical concentration of two water soluble, but mutually incompatible, components (Albertsson 1986). This technique is now recognized as an alternative for the fractionation of biomolecules due to economic advantages and technological simplicity (Rosa et al 2010, Espitia-Saloma et al 2014. Additionally, ATPS could be considered as an integrated process, in which the insoluble components can be removed while at the same time the target product is purified (Rosa et al 2010).…”

“…The most commonly used systems are based on polymer-polymer or polymer-salt. These systems have some drawbacks such as the high cost of polymers, the low segregation of the phases and the complications associated with recycling components lar for recovery of biomolecules have been studied (Espitia-Saloma et al 2014). Particulary, ATPS formed by alcohol and salts are of low cost and allow an easy recovery of the alcohol by evaporation or distillation (Reis et al 2014).…”

“…Aqueous two-phase system (ATPS) extraction is a liquid-liquid extraction technique that has been used for the extraction and partial purification of a wide variety of biomolecules (Rosa et al 2010, Espitia-Saloma et al 2014, Reis et al 2014, Wu et al 2014. ATPS form when an aqueous solution exceeds a critical concentration of two water soluble, but mutually incompatible, components (Albertsson 1986).…”