volume 50, issue 4, P457-467 2007
DOI: 10.1002/jctb.280500404
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Abstract: A B S T R A C TAutomated assay techniques are described for on-line measurements of fumarase activity and total protein concentration, including in-line sample dilution and sample multiplexing during continuous aqueous phase extraction. Fumarase was determined by following the conversion of L-malate to fumurate at a wavelength of 250 nm, while the protein assay was based on the Biuret reaction. Actual assay times of 2and 4 min were achieved for the fumarase and protein measurements, respectively, with an effe…

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