Content aware image restoration improves spatiotemporal resolution in luminescence imaging

Abstract

Luminescent reporters are due to their intrinsically high signal-to-noise ratio a powerful labelling tool for microscopy and macroscopic in vivo imaging in biomedical research. However, luminescence signal detection requires longer exposure times than fluorescence imaging and is consequently less suited for applications requiring high temporal resolution or throughput. Here we demonstrate that content aware image restoration can drastically reduce the exposure time requirements in luminescence imaging, thus overcoming one of the major limitations of the technique.

Epidermal turnover in the planarianSchmidtea mediterraneainvolves basal cell extrusion and intestinal digestion

“…Live imaging planarians remains a challenge and planarian cell dynamics have so far not been visualized. Building on our previous efforts (Boothe et al, 2017; Boothe et al, 2023), we developed a live imaging procedure involving the combination of Linalool anaesthetization and agarose embedding under a gas permeable Polymethylpentene (thermoplastic polyolefin) disc to immobilize the worms. Besides, low-excitation imaging of far-red live dyes was implemented to minimize the strong photosensitivity of planarians especially to shorter wavelengths (Shettigar et al, 2017) and post-acquisition AI-denoising (Fig.…”