Consumption of polyunsaturated fat improves the saturated fatty acid‐mediated impairment of HDL antioxidant potential

Abstract

SFA intake impairs the antioxidant potential of HDL and increases serum levels of oxidized lipoprotein species whereas the antioxidant potential of HDL is enhanced after PUFA consumption.

ApoA-I mimetic administration, but not increased apoA-I-containing HDL, inhibits tumour growth in a mouse model of inherited breast cancer

“…Oxidation kinetics were followed by continuous monitoring of the formation of conjugated dienes50. After 2.5 μM CuSO 4 was added to the lipoproteins, continuous monitoring was performed at 234 nm absorbance (BioTek Synergy, Winooski, VT) at 37 °C for 4 h, using a 96-microwell plate for UV detection (Greiner, Hannover, Germany).…”

“…After 2.5 μM CuSO 4 was added to the lipoproteins, continuous monitoring was performed at 234 nm absorbance (BioTek Synergy, Winooski, VT) at 37 °C for 4 h, using a 96-microwell plate for UV detection (Greiner, Hannover, Germany). The lag phase was calculated as described previously50. The kinetics of LDL in LDL+HDL incubations was calculated by subtracting the kinetics of HDL incubated without LDL from the total kinetics.…”

The interaction between dietary marine components and intestinal flora

“…Notably, the biggest difference between marine lipids and terrestrial lipids is that marine lipids are rich in polyunsaturated fatty acids (PUFA) especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) (Cedó et al 2015). Accumulating evidence has shown that marine lipids exhibit significant efficacy in preventing hyperlipidemia, nonalcoholic fatty acid, diabetes, cancers, inflammatory, and neurodegenerative diseases.…”

Phytosterol-mediated inhibition of intestinal cholesterol absorption in mice is independent of liver X receptor

“…Total RNA from liver and small intestine was extracted using TRIzol LS Reagent (Invitrogen, Carlsbad, CA) following the manufacturer's instructions, and purified using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). cDNA was generated using Oligo(dT) 23 and dNTPs mix (Sigma-Aldrich) and M-MLV Reverse Transcriptase RNase H Minus Point Mutant (Promega, Madison, WI), and was subjected to quantitative real-time PCR amplification using the TaqMan Master Mix (Applied Biosystems, Foster City, CA) and specific TaqMan probes (Applied Biosystems) for abca1 (Mm004426464_m1), abcg5 (Mm00446241_m1), abcg8 (Mm00445970_m1), acaca (Mm01304257_m1), cyp7a1 (Mm00470430_m1), fasn (Mm01253292_m1), npc1l1 (Mm01191972_m1), nr1h3 (Mm00443451_m1), and gapdh (Mm99999915_g1) as the internal control gene. Reactions were run on a CFX96TM Real-Time System (Bio-Rad, Hercules, CA) according to the manufacturer's instructions.…”