Consumption of a Mango Fruit Powder Protects Mice from High-Fat Induced Insulin Resistance and Hepatic Fat Accumulation

Sabater, Agustín G.; Ribot, Joan; Priego, Teresa; Vazquez, I. Alonso; Frank, S.; Palou, Andreu; Buchwald-Werner, Sybille

doi:10.1159/000477606

Cited by 17 publications

(14 citation statements)

References 61 publications

(72 reference statements)

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“…Obesity is also known to cause insulin resistance, which serves a key role in type 2 diabetes. Previous studies have indicated that in mice fed with HFD, an increase in fasting blood glucose and insulin levels was induced, while plant extracts were demonstrated to suppress the HFD-induced increase in these levels in obese animals (27)(28)(29). In the present study, the HFD also led to an increase in serum glucose and insulin levels and this increase in glucose and insulin levels were markedly decreased when mice fed with the HFD were administered dLE.…”

Section: Discussionsupporting

confidence: 61%

Anti-obesity effects of Diospyros�lotus leaf extract in mice with high-fat diet-induced obesity

Kim

Cho²,

Jang³

2018

Int J Mol Med

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Diospyros (D.) lotus has been demonstrated to have antioxidant and anti-inflammatory properties. The purpose of the present study was to evaluate the effect of D. lotus leaf water extract (DLE) on high-fat diet (HFD)-induced obesity in c57BL/6 mice. The present study first investigated the effect of DLE on the lipid accumulation and triglyceride (TG) contents in 3T3-L1 cells, and the results revealed that treatment with DLE suppressed the lipid accumulation and TG level. Subsequently, the anti-obesity effects of DLE were investigated in vivo. Oral administration of DLE reduced the body weight gain, food efficiency ratio, and liver and visceral fat weight in mice fed with a HFD. DLE administration in these mice also reduced TG, total cholesterol, low-density lipoprotein cholesterol, glucose, insulin and leptin levels, as well as the atherogenic index. Furthermore, DLE administration decreased hepatic steatosis, as well as serum aspartate transaminase, alanine transaminase and alkaline phosphatase levels in mice fed with HFD. It was further observed that treatment of the HFD-fed mice with DLE prevented lipid peroxidation, while it recovered glutathione depletion and the activities of superoxide dismutase, catalase and glutathione peroxidase. In conclusion, the current study suggests that the anti-obesity effect of DLE may provide positive insights as a potential functional food ingredient for the prevention of obesity.

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Section: Discussionsupporting

confidence: 61%

Anti-obesity effects of Diospyros�lotus leaf extract in mice with high-fat diet-induced obesity

Kim

Cho²,

Jang³

2018

Int J Mol Med

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“…A previous study demonstrated acetic acid activates AMPKα, which in turn upregulates the expression of lipid oxidation genes in the liver to reduce fat accumulation [16]. Liver-specific AMPKα deletion in mice leads to increased plasma TG content and hepatic lipogenesis [17].…”

Section: Discussionmentioning

confidence: 99%

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

Xiao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Acetic acid (AcOH), a short-chain fatty acid, is reported to have some beneficial effects on metabolism. Therefore, the aim of this study was to investigate the regulatory mechanism of acetic acid on hepatic lipid metabolism in BRL-3A cells. Methods: We cultured and treated BRL-3A cells with different concentrations of sodium acetate (neutralized acetic acid) and BML-275 (an AMPKα inhibitor). The total lipid droplet area was measured by oil red O staining, and the triglyceride content was determined by a triglyceride detection kit. We detected mRNA and protein levels of lipid metabolism-related signalling molecules by RT-PCR and Western blot. Results: Acetic acid treatment increased AMPKα phosphorylation, which subsequently increased the expression and transcriptional activity of peroxisome proliferator-activated receptor α and upregulated the expression of lipid oxidation genes. These changes ultimate led to increasing levels of lipid oxidation in BRL-3A cells. Furthermore, elevated AMPKα phosphorylation reduced the expression and transcriptional activity of the sterol regulatory element-binding protein 1c, which reduced the expression of lipogenic genes, thereby decreasing lipid biosynthesis in BRL-3A cells. Consequently, triglyceride content in acetate-treated BRL-3A cells was significantly decreased. Conclusions: These results indicate that acetic acid activates the AMPKα signalling pathway, leading to increased lipid oxidation and decreased lipid synthesis in BRL-3A cells, thereby reducing liver fat accumulation in vitro.

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“…Bovine lactadherin, free of lipid and protein contaminants, was purified and labeled with Alexa Fluor 647 or Alexa Fluor 488 [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

Procoagulant Activity of Blood and Endothelial Cells via Phosphatidylserine Exposure and Microparticle Delivery in Patients with Diabetic Retinopathy

Chen

Dong

et al. 2018

Cell Physiol Biochem

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Background/Aims: The mechanisms for thrombosis in diabetic retinopathy (DR) are complex and need to be further elucidated. The purpose of this study was to test phosphatidylserine (PS) exposure on microparticles (MPs) and MP-origin cells from the circulation and to analyze cell-/MP-associated procoagulant activity (PCA) in DR patients. Methods: PS-positive MPs and cells from healthy controls (n = 20) and diabetic patients (n = 60) were analyzed by flow cytometry and confocal microscopy. Clotting time and purified coagulation complex assays were used to measure PCA. Results: PS exposure on platelets and monocytes was higher in proliferative DR (PDR) patients than in non-PDR patients or controls. The highest levels of MPs (derived from platelets [30%], erythrocytes [13%], leukocytes [28%], and endothelial cells [10%]) were found in patients with PDR. In addition, PS exposure on blood cells and shed MPs in DR patients led to significantly increased FXa and FIIa generation, fibrin formation, and markedly shortened coagulation time. Moreover, lactadherin reduced 70% of PCA by blocking PS, while an anti-tissue factor antibody had a smaller effect. Conclusion: Our results confirmed that PCA in DR patients may be partly ascribed to PS exposure and MP release from blood and endothelial cells. Lactadherin may act as an efficient anticoagulant factor in this process.

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Consumption of a Mango Fruit Powder Protects Mice from High-Fat Induced Insulin Resistance and Hepatic Fat Accumulation

Cited by 17 publications

References 61 publications

Anti-obesity effects of Diospyros�lotus leaf extract in mice with high-fat diet-induced obesity

Anti-obesity effects of Diospyros�lotus leaf extract in mice with high-fat diet-induced obesity

Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway

Procoagulant Activity of Blood and Endothelial Cells via Phosphatidylserine Exposure and Microparticle Delivery in Patients with Diabetic Retinopathy

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