Construction of RFLP-based maps of foxtail millet, Setaria italica (L.) P. Beauv.

Wang, Z. M.; Devos, Katrien M.; Liu, C. J.; Wang, R. Q.; Gale, M. D.

doi:10.1007/s001220050705

Cited by 113 publications

(106 citation statements)

References 24 publications

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“…A cross between S. italica inbred B100 and S. viridis accession A10 was performed in 1997, and an F2 generation of this cross was used to generate the first genetic map of Setaria italica/viridis 11,24 . We used 247 progeny of this cross to construct a recombinant inbred line (RIL) population through eight generations of singleseed descent.…”

Section: A Genetic Map For Setariamentioning

confidence: 99%

Reference genome sequence of the model plant Setaria

et al. 2012

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Section: A Genetic Map For Setariamentioning

confidence: 99%

Reference genome sequence of the model plant Setaria

et al. 2012

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“…Foxtail millet is closely related to several biofuel grasses with complex genomes, such as switchgrass (Panicum virgatum), napier grass (Pennisetum purpureum) and pearl millet (Pennisetum glaucum). Of these related species, foxtail millet is the most suitable plant for whole genome sequencing and analysis owing to its small genome (~490 Mb) 2 , self-pollination, rich genetic diversity (~6,000 varieties) 3,4 , the existence of a complete collection of germplasm and the availability of high-throughput transformation platforms 2 .…”

Section: A R T I C L E Smentioning

confidence: 99%

“…Genetic maps have already been constructed for foxtail millet using different markers 3,4 . Comparisons between genetic maps of foxtail millet, rice and pearl millet have shed light on the chromosomal relationships between these grasses 4,5 .…”

Section: A R T I C L E Smentioning

confidence: 99%

“…Carboxylase, which catalyses the first reaction in C 4 photosynthesis, is thought to differ in C 3 and C 4 plants 14 . However, evolution of carboxylase genes in C 3 and C 4 plants is still not well understood, especially in grasses. Using the foxtail millet genome draft, we were able to analyze carboxylase gene evolution in C 3 and C 4 grasses.…”

Section: A R T I C L E Smentioning

confidence: 99%

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Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential

et al. 2012

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“…As an alternative method, mature panicles can be dipped in warm water to heat-kill developing pollen grains 9,10,13,14 with the advantage of performing crosses on a large number of panicles. However, the temperature and duration of heat treatment varies widely from different experiments (e.g.…”

Section: Introductionmentioning

confidence: 99%

Methods for Performing Crosses in <em>Setaria viridis</em>, a New Model System for the Grasses

Jiang

Barbier²,

Brutnell

2013

JoVE

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Setaria viridis is an emerging model system for C 4 grasses. It is closely related to the bioenergy feed stock switchgrass and the grain crop foxtail millet. Recently, the 510 Mb genome of foxtail millet, S. italica, has been sequenced 1,2 and a 25x coverage genome sequence of the weedy relative S. viridis is in progress. S. viridis has a number of characteristics that make it a potentially excellent model genetic system including a rapid generation time, small stature, simple growth requirements, prolific seed production 3 and developed systems for both transient and stable . However, the genetics of S. viridis is largely unexplored, in part, due to the lack of detailed methods for performing crosses. To date, no standard protocol has been adopted that will permit rapid production of seeds from controlled crosses.The protocol presented here is optimized for performing genetic crosses in S. viridis, accession A10.1. We have employed a simple heat treatment with warm water for emasculation after pruning the panicle to retain 20-30 florets and labeling of flowers to eliminate seeds resulting from newly developed flowers after emasculation. After testing a series of heat treatments at permissive temperatures and varying the duration of dipping, we have established an optimum temperature and time range of 48 °C for 3-6 min. By using this method, a minimum of 15 crosses can be performed by a single worker per day and an average of 3-5 outcross progeny per panicle can be recovered. Therefore, an average of 45-75 outcross progeny can be produced by one person in a single day. Broad implementation of this technique will facilitate the development of recombinant inbred line populations of S. viridis X S. viridis or S. viridis X S. italica, mapping mutations through bulk segregant analysis and creating higher order mutants for genetic analysis.

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Construction of RFLP-based maps of foxtail millet, Setaria italica (L.) P. Beauv.

Cited by 113 publications

References 24 publications

Reference genome sequence of the model plant Setaria

Reference genome sequence of the model plant Setaria

Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential

Methods for Performing Crosses in <em>Setaria viridis</em>, a New Model System for the Grasses

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