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Cited by 136 publications
(104 citation statements)
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“…The B.subtilis yclNd, yclPd, and yclQd mutant strains were complemented with a copy of the corresponding gene under the control of the IPTG-inducible Pspac promoter using vector pHCMC05 (Bacillus Genetic Stock Center) (23). The complete yclQ, yclN, and yclP were amplified from B. subtilis chromosomal DNA by PCR with primers introducing BamHI and XbaI restriction sites (Table S1).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The B.subtilis yclNd, yclPd, and yclQd mutant strains were complemented with a copy of the corresponding gene under the control of the IPTG-inducible Pspac promoter using vector pHCMC05 (Bacillus Genetic Stock Center) (23). The complete yclQ, yclN, and yclP were amplified from B. subtilis chromosomal DNA by PCR with primers introducing BamHI and XbaI restriction sites (Table S1).…”
Section: Methodsmentioning
confidence: 99%
“…In the disc diffusion assay, 3,4-DHB did not relieve iron limitation imposed by 2,2Ј-dipyridyl. Once the disrupted genes were complemented with the copies of the respective transporter genes on the IPTG-inducible expression vector pHCMC05 (23), the uptake of radioactive ferric complexes of PB, its photoproduct and 3,4-DHB was largely restored (Fig. 2 B, D, and F).…”
mentioning
confidence: 99%
“…Plasmid pAJ018 was constructed using PCR to amplify the nucleotide sequence in pAJ001 including the ribosomal binding site and tagged E. coli dgkA. The forward primer contained a BamHI site, and the reverse primer contained an XbaI site downstream of the stop codon, and the product was ligated into the B. subtilis expression vector pHCMC02 (23).…”
Section: Methodsmentioning
confidence: 99%
“…The recombinant plasmid construction was confirmed by restriction analysis and automated DNA sequencing and named pLDVP1. It expresses the Stx2B subunit, accumulated as an intracellular protein, under the control of the stress inducible sigma B-dependent promoter derived from the B. subtilis gsiB gene (15). The pLDVP1 plasmids were replicated in competent E. coli DH5a cells and, then, transferred to B. subtilis WW02.…”
Section: Plasmid Constructionmentioning
confidence: 99%