Construction of neurospheroids via surface modified concave microwells

Lee, Geon Hui; Lim, Jaeho; Park, Ji Soo; Lee, Wonseok; Yoon, Dae Sung; Kim, Soo Hyun; Kim, Myung Ki; Lee, Sang Hoon; Kim, Dong Hwee

doi:10.1016/j.jiec.2018.01.014

Cited by 8 publications

(7 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we found that APTES alone was capable of mobilizing Ca 2+ in MG-63s even more than PPAAm -surfaces that do not present integrin ligands. Lee et al (2018) presented ATPES-treated PDMS concave microwell surfaces to cortical neurons which revealed optimal surface conditions additionally supporting a spheroid formation. The authors also stimulated the cells with KCl for spontaneous calcium transients (fluo-4 AM), observing typical temporal Ca 2+ responses in all chemically modified microwells based on APTES (APTES, APTES-Laminin, APTES-poly-L-lysine, APTES-carbon nanotubes).…”

Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 93%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.

show abstract

Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 93%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Many tissue engineering studies have been carried out with various cell culture plates 14,15 . However, these studies have limited access to control the geometry of the cell culture plates.…”

Section: Introductionmentioning

confidence: 99%

Design and manufacture of 3D cell culture plate for mass production of cell-spheroids

Lee

Pathak

Jeong

2019

Sci Rep

View full text Add to dashboard Cite

A 3D cell culture is preferred to 2D cell culture since it allows cells to grow in all directions in vitro, similar to how they would in vivo. 3D cell culture plates currently used in tissue engineering research have limited access to control the geometry. Furthermore, 3D cell culture plate manufacturing methods are relatively complex, time-consuming, labor-intensive, and expensive. Therefore, a design and manufacturing method, which has relatively low cost, high throughput, and high size flexibility, is proposed. Cell culture plate was fabricated by computer aided design and manufacturing software using polydimethylsiloxane as a plate constituent. With the successfully-developed 3D cell culture plate, the morphology and viability of the cultured mesenchymal stem cells were tested.The mesenchymal stem cells seeded on the newly-fabricated 3D cell culture plate aggregated to form 3D spheroids within 24 h of incubation and well-maintained their viability. Thus, due to the capacity of mass production of the cell spheroids with a desired cell viability, the newly-fabricated plate has a great promise to prepare 3D cell spheroids for experimental as well as clinical applications.

show abstract

“…According to the above Equation (2), air pocket entrapment occurs when the aspect ratio of the concave structure becomes larger than the k value determined by the contact angle θ. Since the water contact angle θ of PDMS is known to be 99.2 ± 2.27° [45], the aspect ratio k is approximately 0.04. Therefore, air pocket entrapment occurs when the 100 μm-width concave structure has a depth of more than 4 μm.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Convex PDMS–Parylene Microstructures for Conformal Contact of Planar Micro-Electrode Array

Lee

Park

et al. 2019

Polymers

View full text Add to dashboard Cite

Polymer-based micro-electrode arrays (MEAs) are gaining attention as an essential technology to understand brain connectivity and function in the field of neuroscience. However, polymer based MEAs may have several challenges such as difficulty in performing the etching process, difficulty of micro-pattern generation through the photolithography process, weak metal adhesion due to low surface energy, and air pocket entrapment over the electrode site. In order to compensate for the challenges, this paper proposes a novel MEA fabrication process that is performed sequentially with (1) silicon mold preparation; (2) PDMS replica molding, and (3) metal patterning and parylene insulation. The MEA fabricated through this process possesses four arms with electrode sites on the convex microstructures protruding about 20 μm from the outermost layer surface. The validity of the convex microstructure implementation is demonstrated through theoretical background. The electrochemical impedance magnitude is 204.4 ± 68.1 kΩ at 1 kHz. The feasibility of the MEA with convex microstructures was confirmed by identifying the oscillation in the beta frequency band (13–30 Hz) in the electrocorticography signal of a rat olfactory bulb during respiration. These results suggest that the MEA with convex microstructures is promising for applying to various neural recording and stimulation studies.

show abstract

Construction of neurospheroids via surface modified concave microwells

Cited by 8 publications

References 66 publications

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Design and manufacture of 3D cell culture plate for mass production of cell-spheroids

Fabrication of Convex PDMS–Parylene Microstructures for Conformal Contact of Planar Micro-Electrode Array

Contact Info

Product

Resources

About