DOI: 10.1007/978-1-4939-7435-1_15
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Abstract: MicroRNA is a small, endogenous RNA that inhibits specific gene expression by interacting mostly on the UTR region of target mRNA. Among various methods to inhibit the microRNA, microRNA sponge is a construct designed to inhibit specific microRNA by providing excess amount of target mRNA. Here, we describe a method to generate multi-potent miRNA sponge which can inhibit multiple microRNAs simultaneously. In addition, two methods to examine the functionality of miRNA sponge will be introduced. This tool can be …

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