Construction of liver tissue in vivo with preparative partial hepatic irradiation and growth stimulus: investigations of less invasive techniques and progenitor cells

Miyazaki, Keiko; Yamanouchi, Kaoru; Sakai, Yusuke; Yamaguchi, Izumi; Takatsuki, Mitsuhisa; Kuroki, Tamotsu; Guha, Chandan; Eguchi, Susumu

doi:10.1016/j.jss.2013.06.016

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…This correlates well with clinical data where the volume of liver receiving more than 30 Gy is associated with the risk of RILD [5]. This model provides a significant improvement over previous pre-clinical liver irradiation techniques that require either laparotomy or the use of larger rodent models such as rats [6,7,8,16].…”

Section: Discussionsupporting

confidence: 77%

Non-Invasive Targeted Hepatic Irradiation and SPECT/CT Functional Imaging to Study Radiation-Induced Liver Damage in Small Animal Models

Kabarriti

Brodin

Yaffe

et al. 2019

Cancers

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Radiation therapy (RT) has traditionally not been widely used in the management of hepatic malignancies for fear of toxicity in the form of radiation-induced liver disease (RILD). Pre-clinical hepatic irradiation models can provide clinicians with better understanding of the radiation tolerance of the liver, which in turn may lead to the development of more effective cancer treatments. Previous models of hepatic irradiation are limited by either invasive laparotomy procedures, or the need to irradiate the whole or large parts of the liver using external skin markers. In the setting of modern-day radiation oncology, a truly translational animal model would require the ability to deliver RT to specific parts of the liver, through non-invasive image guidance methods. To this end, we developed a targeted hepatic irradiation model on the Small Animal Radiation Research Platform (SARRP) using contrast-enhanced cone-beam computed tomography image guidance. Using this model, we showed evidence of the early development of region-specific RILD through functional single photon emission computed tomography (SPECT) imaging.

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Section: Discussionsupporting

confidence: 77%

Non-Invasive Targeted Hepatic Irradiation and SPECT/CT Functional Imaging to Study Radiation-Induced Liver Damage in Small Animal Models

Kabarriti

Brodin

Yaffe

et al. 2019

Cancers

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“…Surgical options for providing mitotic stimulation include occlusion of a portal vein branch [18] . , which can be permanent [19,20] or reversible [21], and partial hepatectomy [8,[22][23][24][25][26][27][28]. To circumvent the limitation of these invasive procedures, pharmacological approaches have been explored as an alternative.…”

Section: Promoting Proliferation Of the Engrafted Hepatocytesmentioning

confidence: 99%

“…We have demonstrated that preparative HIR delivered during laparotomy combined with the expression of hepatic growth factor in the liver resulted in massive repopulation of the liver with transplanted hepatocytes (Table A) [13,19,28,33,38]. We first examined the potential of hepatocyte transplantation in ameliorating radiation induced liver disease (RILD) in F344 rats subjected to partial hepatectomy and whole liver radiation therapy.…”

Section: Preparative Regimen Of Hir For Htmentioning

confidence: 99%

Hepatocyte Transplantation: Quo Vadis?

Barahman

Asp

Roy‐Chowdhury

et al. 2019

International Journal of Radiation Oncology*Biology*Physics

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Orthotopic liver transplantation (OLT) has been effective in managing end-stage liver disease since the advent of Cyclosporine immunosuppression therapy in 1980. The major limitations of OLT are organ supply, monetary cost and burden of lifelong immunosuppression. Hepatocyte transplantation (HT), as a substitute for OLT, has been an exciting topic of investigation for several decades. HT is potentially minimally invasive, and can also serve as a vehicle for delivery of personalized medicine through autologous cell transplant after modification ex vivo. However, three major hurdles have prevented large-scale clinical application: (a) availability of transplantable cells; (b) safe and efficient ex vivo gene therapy methods, and (c) engraftment and repopulation efficiency. This review will discuss new sources for transplantable liver cells obtained by lineage-reprogramming, clinically acceptable methods of genetic manipulation, and the development of hepatic irradiation (HIR)-based preparative regimens for enhancing engraftment

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“…The cells are injected into the recipient through portal vein, peripheral vein [30], and intraspleenic [32] or intraperitoneal route. To enhance the transplantation efficiency, conditioning of recipient liver with partial hepatectomy [33,34], liver irradiation [35,36], or portal embolization [37] has been recently proposed. Broadly, cells are categorized into two main categories; stem cells and mature hepatocytes are the potential cell-based therapies adapted to date in the cure and regeneration of liver cirrhosis [5].…”

Section: Cell-based Therapies For Regeneration Of Liver Cirrhosismentioning

confidence: 99%

Regenerative Medicine in Liver Cirrhosis: Promises and Pitfalls

Tayyeb¹,

Azam²,

Nisar³

et al. 2017

Liver Cirrhosis - Update and Current Challenges

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Liver cirrhosis is irreversible and mostly ends up with complete loss of liver function/ end-stage liver failure, and the only proven treatment is liver transplantation. Scarcity of donor, high cost, lifelong immunosuppression, and surgical complications are the major issues associated with liver transplantation and these urge to look for alternate therapeutic approaches. Advancements in the field of regenerative medicine are arising hope for the treatment of liver cirrhosis. This chapter deals with the scope of liver regenerative medicine in the treatment of liver cirrhosis. Review of the literature showed that liver regenerative medicine no doubt holds great promises and added a lot of hope to the cure of liver diseases. Primarily, cell-based therapies had shown great potential to treat liver cirrhosis. Successful clinical human trials further strengthen their significance in the field. However, recent trends in liver regenerative medicine are focusing on the development of tissue engineering leading to generation of the whole organ. Despite advantages, liver regenerative medicine has several limitations and sometimes been over-optimistically interpreted. In conclusion, the current scenario advocates to conduct more preclinical and clinical trials to effectively replace liver transplantation with liver regenerative medicine to treat liver diseases.

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Construction of liver tissue in vivo with preparative partial hepatic irradiation and growth stimulus: investigations of less invasive techniques and progenitor cells

Abstract: Background: The selective proliferation of transplanted hepatocytes by providing a

Cited by 4 publications

References 26 publications

Non-Invasive Targeted Hepatic Irradiation and SPECT/CT Functional Imaging to Study Radiation-Induced Liver Damage in Small Animal Models

Non-Invasive Targeted Hepatic Irradiation and SPECT/CT Functional Imaging to Study Radiation-Induced Liver Damage in Small Animal Models

Hepatocyte Transplantation: Quo Vadis?

Regenerative Medicine in Liver Cirrhosis: Promises and Pitfalls

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