Construction of an ultrasensitive non-enzymatic sensor to investigate the dynamic process of superoxide anion release from living cells

Polymer-Encapsulated Cobalt/Gold Bimetallic Nanoclusters as Stimuli-Responsive Chemiluminescent Nanoprobes for Reactive Oxygen Species

“…Great efforts have been devoted to exploring reliable ROS analytical methodologies. − In particular, enzyme-based methods have received considerable attention because of good selectivity and high sensitivity . Nevertheless, enzyme-based methods are often accompanied by inherent disadvantages such as relatively high cost, short lifetime, poor reproducibility, complicated immobilization procedure, and critical operating situation, which remain as pivotal problems for applications . In contrast, nonenzymatic methods are preferred because they show conveniences and benefits to avoid the weaknesses of enzymatic methods.…”

“…7 Nevertheless, enzyme-based methods are often accompanied by inherent disadvantages such as relatively high cost, short lifetime, poor reproducibility, complicated immobilization procedure, and critical operating situation, which remain as pivotal problems for applications. 8 In contrast, nonenzymatic methods are preferred because they show conveniences and benefits to avoid the weaknesses of enzymatic methods. But, the sensitivity and selectivity of nonenzymatic methods cannot fully meet the detection requirements in practical biological samples.…”

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

“…[ [116][117][118] To improve overall sensing elements, methods to control the morphology and distribution of inorganic materials as sensing elements could be further developed. [ 115,283,284] It provides long-term stability with excellent reusability.…”

Investigation of the Relationship Between H2O2 and HClO in Living Cells by a Bifunctional, Dual-ratiometric Responsive Fluorescent Probe

“…As the important species within and between cells, reactive oxygen species (ROS), such as O 2 · – , ·OH, 1 O 2 , HClO, and H 2 O 2 , play vital roles in cell signal transmission, differentiation, migration, cellular immunity, and body’s defense against pathogens. − H 2 O 2 , as the most described signaling molecule among ROS families, is relatively stable and has various indispensable functions in cell signal transduction and homeostasis. − H 2 O 2 is a precursor and a byproduct in ROS chain reactions, and its excessive production can lead to oxidative damage and thereby causing aging and various diseases including cardiovascular diseases, neurodegenerative diseases, inflammation, cancer, etc. − Strong oxidant HClO is a microbicidal effector to cope with microbial invasion in immune system. , The abnormal accumulation of HClO can cause serious tissue damage to induce a series of diseases including neurodegenerative diseases, atherosclerosis, cancer, ischemia, reperfusion injury, rheumatoid rheumatism, and so on. − In many cases, the delicate balance between oxidative stress and signal transduction in cells is strictly manipulated by a group of reactive oxygen species, which are mutually dependent and are often transformed into each other through intracellular reactions . Since ROS are very reactive and their intracellular concentrations vary greatly, particularly in physiological and pathological conditions, the simultaneous investigation of the activity of multiple ROS in cells is critically important to help study their interactions and synergistic effects in complex environments.…”