Construction of a Tn5 derivative encoding bioluminescence and its introduction in Pseudomonas, Agrobacterium and Rhizobium

Boivin, Rodolphe; Chalifour, François-P.; Dion, Patrice

doi:10.1007/bf00333397

Cited by 45 publications

(32 citation statements)

References 23 publications

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“…To study the patterns of microbial plant root colonization, different tools such as microscopy (Chin-A-Woeng et al 1997;Rovira et al 1974), microscopy combined with the use of marked strains (Bloemberg et al 1997), or strains equipped with reporter genes (Beauchamp et al 1993;Boivin et al 1988;de Weger et al 1991;Kloepper and Beauchamp 1992) can be used. During in vitro studies, it was shown that, upon inoculation of seed or seedlings, cells of Pseudomonas mainly appear as microcolonies along the junctions of epidermal plant cells where nutrients are exuded Chin-A-Woeng et al 1997).…”

Section: Rhizoremediationmentioning

confidence: 99%

Rhizoremediation: A Beneficial Plant-Microbe Interaction

Kuiper¹,

Lagendijk²,

Bloemberg³

et al. 2004

MPMI

740

394

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Worldwide, contamination of soil and ground water is a severe problem. The negative effects of pollutants on the environment and on human health are diverse and depend on the nature of the pollution. The search for alternative methods for excavation and incineration to clean polluted sites resulted in the application of bioremediation techniques. In this review, we describe some generally accepted bioremediation tools and subsequently focus on the combination of two approaches, phytoremediation and bioaugmentation, resulting in rhizoremediation. During rhizoremediation, exudates derived from the plant can help to stimulate the survival and action of bacteria, which subsequently results in a more efficient degradation of pollutants. The root system of plants can help to spread bacteria through soil and help to penetrate otherwise impermeable soil layers. The inoculation of pollutant-degrading bacteria on plant seed can be an important additive to improve the efficiency of phytoremediation or bioaugmentation.

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Section: Rhizoremediationmentioning

confidence: 99%

Rhizoremediation: A Beneficial Plant-Microbe Interaction

Kuiper¹,

Lagendijk²,

Bloemberg³

et al. 2004

MPMI

740

394

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“…To complete the series, mini-Tn5xylE and mini-TnSluxAB elements were developed also. These produce type I fusions to catechol 2,3-dioxygenase of Pseudomonas putida (34) and to the luciferase genes of Vibrio harveyi, respectively (3,7,17). These two reporter genes are convenient alternatives to lacZ in a variety of applications (5).…”

mentioning

confidence: 99%

Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria

et al. 1990

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“…Two advantages of this approach, beyond the reduction in size of the transposon insert, are that it prevents further transposition and that it permits additional rounds of mini transposon mutagenesis because no immunity protein is present in the cell [11]. Whereas TnphoA and Tnlacz can be used to explore membrane protein topology, TnluxAB encodes bioluminescence and can be used to visualize bacterial presence for example in an inoculated Rhizobial strain [12].…”

Section: Discussionmentioning

confidence: 99%

TnphoA and Its Use in Transposon Mutagenesis

Das¹,

Nester²

2014

AiM

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TnphoA construct was derived from the transposon Tn5, which contained a kanamycin resistance gene flanked by two IS50 elements. This construct contains a version of the alkaline phosphatase gene with its signal sequence and promoter deleted, which will result in a blue colony phenotype on X-phos (5-bromo-4-chloro-3-indolyl phosphate toluidine salt) containing media when secreted into the periplasm. This secretion can occur only if the TnphoA has been inserted into a gene with a signal sequence which directs that gene-product to leave the cytosol. Additionally, the transposon must be inserted into the correct orientation and the same reading frame as the gene has been inserted into. TnphoA's transposition activity derives from that of this Tn5 transposon by a conservative mechanism in a relatively rare but highly regulated process. The phoA portion of the TnphoA construct came from Escherichia coli K12 where it coded for the periplasmic protein alkaline phosphatase. This enzyme must be secreted from the cytoplasm in order to demonstrate activity and its secretion is determined by the presence of a signal sequence at its amino-terminal end. TnphoA mutagenesis is the identification of new genes that code for transmembrane or secreted proteins. TnphoA is an extremely useful construct, which along with other derivatives of the transposon Tn5 is used extensively in transposon mutagenesis based genetic analysis. TnphoA will ensure its continued significance and prominence in the area of transposon mutagenesis. TnphoA has been used to isolate new chromosomal genes, whose products are secreted, as in the case of some virulence genes in Agrobacterium tumefaciens and symbiotic genes in Rhizobium meliloti.

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Construction of a Tn5 derivative encoding bioluminescence and its introduction in Pseudomonas, Agrobacterium and Rhizobium

Cited by 45 publications

References 23 publications

Rhizoremediation: A Beneficial Plant-Microbe Interaction

Rhizoremediation: A Beneficial Plant-Microbe Interaction

Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria

TnphoA and Its Use in Transposon Mutagenesis

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