Construction of a synthetic immunogen: use of the natural immunomodulator polytuftsin in malaria vaccines against RESA antigen of Plasmodium falciparum

Pawan, Kumar; Ivanov, Boris; Kabilan, L; Rao, D. N.

doi:10.1016/0264-410x(94)90291-7

Cited by 14 publications

(9 citation statements)

References 24 publications

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“…The in vitro merozoite reinvasion inhibition assay was performed as described earlier [24]. In brief, the original cultures (5-10% parasitemia) of Plasmodium falciparum (Indian FJB-D9 strain) were synchronized by sorbitol (5%) lysis to select for late trophozoite and schizonts stages.…”

Section: In Vitro Merozoite Reinvasion Inhibition Assaymentioning

confidence: 99%

Bioactive fragment of human interleukin-1 b (163-171) modulates the immune response to synthetic peptides of RESA of Plasmodium falciparum

Thomas

Prasad

Gokulan

et al. 1999

Medical Microbiology and Immunology

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In this study a bioactive fragment, residues 163-171 of human interleukin-1beta (hu IL-1beta), that mimics the functions of the whole IL-1 molecule was chemically linked to two peptide sequences of the ring erythrocyte surface antigen (RESA), an asexual blood stage antigen of Plasmodium falciparum. The immunogenicity of different formulations was studied in different haplotypes of inbred mice. The peptide-IL-1beta conjugates delivered in liposomes showed the maximal antibody production. Antigen entrapped in liposomes at a dose of 5 microg showed antibody levels comparable to that of peptide conjugate in alum. The IgG subclass profile with different RESA peptides and its conjugates at various doses in liposomes induced predominantly IgG2a/2b isotypes while alum-delivered formulations showed both IgG1 and IgG2a/2b isotypes. In vitro studies of merozoite reinvasion showed varying degree (50-85%) of inhibition, maximum being with the peptide conjugates in liposomes (80-87%). Thus, this approach suggests that linking of hu IL-1beta is instrumental in augmenting the immune response against otherwise poorly immunogenic synthetic peptides.

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Section: In Vitro Merozoite Reinvasion Inhibition Assaymentioning

confidence: 99%

Bioactive fragment of human interleukin-1 b (163-171) modulates the immune response to synthetic peptides of RESA of Plasmodium falciparum

Thomas

Prasad

Gokulan

et al. 1999

Medical Microbiology and Immunology

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“…Only the 3' repeat region of RESA has shown the proliferation of T cells from naturally primed individuals, predominantly with 8 X4 mer peptides (4, 15). In the murine system, we reported earlier that 2 X 1 1 mer and 2 X 8 mer peptides failed to show appreciable proliferation (9,10). This lack of effective T-cell help, coupled with genetic restriction of the immune response, might be the causative factor for poor protection seen with RESA antigen-or epitope-based vaccines (3).…”

Section: Cytokinesmentioning

confidence: 99%

Correlation of T‐Cell Response and Lymphokine Profile with RESA Peptides of Plasmodium falciparum Containing a Universal T‐Cell Epitope and an Immunopotentiator, Polytuftsin

Kumar

Rao

1999

Microbiology and Immunology

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“…Several studies indicated that conjugates with polytuftsin retain tuftsin-like effects and increase the epitope specific antibody production. 20,21 These results prompted us to prepare a group of new tuftsin-like biodegradable and immunostimulatory synthetic carriers, which is available for attachment of epitopes in multiple copies resulting in wellcharacterized conjugates. For these sequential oligopeptides with Thr-Lys-Pro-Lys-Gly (TKPKG) sequence were selected.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin‐like sequence

et al. 2004

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Sequential oligopeptides based on a pentapeptide (TKPKG) derived from tuftsin with different lengths were synthesized by stepwise solid phase methodology. These highly soluble oligomers were nontoxic on mouse spleen cells, and other biological data suggested that tuftsin-like properties were also presented. The (TKPKG)n (n=2,4,6,8) oligopeptides were not immunogenic; however, they increased sheep red blood cells (SRBC) antigen specific antibody response in mice, demonstrating their immunostimulatory effect. Chemotactic activity was also found on J774 monocyte cells, while MRC5 fibroblasts were chemotactically nonresponders to the tested forms of tuftsin. These oligomers showed unordered and flexible structure by CD measurements, confirmed by computer modeling studies indicating also a fairly good accessibility of the epsilon-amino group of each lysine residue. Data suggest that these new oligotuftsin derivatives can be considered as promising carriers for synthetic vaccine.

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Construction of a synthetic immunogen: use of the natural immunomodulator polytuftsin in malaria vaccines against RESA antigen of Plasmodium falciparum

Cited by 14 publications

References 24 publications

Bioactive fragment of human interleukin-1 b (163-171) modulates the immune response to synthetic peptides of RESA of Plasmodium falciparum

Bioactive fragment of human interleukin-1 b (163-171) modulates the immune response to synthetic peptides of RESA of Plasmodium falciparum

Correlation of T‐Cell Response and Lymphokine Profile with RESA Peptides of Plasmodium falciparum Containing a Universal T‐Cell Epitope and an Immunopotentiator, Polytuftsin

Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin‐like sequence

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