Construction of a metastasis-associated ceRNA network reveals a prognostic signature in lung cancer

Abstract

Background: Lung cancer is the most common cancer worldwide, and metastasis is the leading cause of lung cancer related death. However, the molecular network involved in lung cancer metastasis remains incompletely described. Here, we aimed to construct a metastasis-associated ceRNA network and identify a lncRNA prognostic signature in lung cancer.Methods: RNA expression profiles were downloaded from The Cancer Genome Atlas (TCGA) database. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and gene set enrichment analysis (GSEA) were performed to investigate the function of these genes. Using Cox regression analysis, we found that a 6 lncRNA signature may serve as a candidate prognostic factor in lung cancer. Finally, we used Transwell assays with lung cancer cell lines to verify that LINC01010 acts as a tumor suppressor.Results: We identified 1249 differentially expressed (DE) mRNAs, 440 DE lncRNAs and 26 DE miRNAs between nonmetastatic and metastatic lung cancer tissues. GO and KEGG analyses confirmed that the identified DE mRNAs are involved in lung cancer metastasis. Using bioinformatics tools, we constructed a metastasis-associated ceRNA network for lung cancer that includes 117 mRNAs, 23 lncRNAs and 22 miRNAs. We then identified a 6 lncRNA signature (LINC01287, SNAP25-AS1, LINC00470, AC104809.2, LINC00645 and LINC01010) that had the greatest prognostic value for lung cancer. Furthermore, we found that suppression of LINC01010 promoted lung cancer cell migration and invasion.Conclusions: This study might provide insight into the identification of potential lncRNA biomarkers for diagnosis and prognosis in lung cancer.

Identification of Five Immune-Related lncRNAs Predicting Survival and Tumor Microenvironment Characteristics in Breast Cancer

“…Various studies have posited that LINC00987 is reduced in patients with ankylosing spondylitis [ 29 ]. LINC01010 has a significant prognostic value that suppresses cancer cell migration and invasion of lung cancer [ 30 ]. ELOVL2-AS1 was also shown to be positively correlated with the survival rate of BRCA patients and may serve as a potential diagnostic or prognostic marker [ 31 ].…”

Hypoxia‐related lncRNAs to build prognostic classifier and reveal the immune characteristics of EGFR wild type and low expression of PD‐L1 squamous and adenocarcinoma NSCLC

“…TUSC8 and LINC01010 are reported to act as a suppressor in the invasion of multiple cancer cells in different pathways. [50][51][52][53] Multiple signatures that predict tumor prognosis also involve LINC01010. [53][54][55] AL161431.1 facilitates tumor cell proliferation and migration in endometrial carcinoma.…”

“…[50][51][52][53] Multiple signatures that predict tumor prognosis also involve LINC01010. [53][54][55] AL161431.1 facilitates tumor cell proliferation and migration in endometrial carcinoma. 56 lncRNAs signature containing AL161431.1 can serve as a prognosis predictor in LUSC.…”