Constructing a high‐density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage

Abstract

To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1 F1 population of Gossypium hirsutum × Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%-2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.

Identification of Chromosome Segment Substitution Lines of Gossypium barbadense Introgressed in G. hirsutum and Quantitative Trait Locus Mapping for Fiber Quality and Yield Traits

“…CSSLs also provide permanent segregation populations for studying multi-environmental stability of the mapped QTLs. In the previous studies, a set of CSSL population was constructed using a widely planted upland cotton cultivar, CCRI36 as the recurrent parent, which is characterized of high yield and early maturity, and a sea island cotton Hai1 as the donor parent, which had good fiber quality and a high level of resistance to Verticillium wilt [38, 39, 40, 41]. In the current study, four introgression lines with excellent fiber quality which derived from this CSSL population were used to construct the segregating populations of double-crossed F 1 , F 2 , and F 2:3 .…”

“…While CCRI36 was a widely grown upland cotton cultivar with high yield and early maturity, and developed by the Institute of Cotton Research of Chinese Academy of Agricultural Sciences (State Approval Certificate of Cotton 990007). The development of the CSSL population was reported by Shi et al [38]. The four BC 5 F 4 introgression lines, MBI9804, MBI9855, MBI9752, and MBI9134, with stable and excellent fiber quality performance, were selected as parental lines to construct a double-crossed population of (MBI9804×MBI9855) × (MBI9752×MBI9134).…”

“…SSR amplification and polyacrylamide gel electrophoresis were performed following Zhang’s description [44]. Based on the previously constructed CCRI36×Hai1 BC 1 F 1 genetic linkage map [38], SSR markers were selected at a distance of 10–20 cM. A total of 526 pairs of polymorphic SSR markers between CCRI36 and Hai1 were selected for screening of polymorphisms among the four parental lines.…”

“…A genetic linkage map containing 2,292 markers was constructed [38] and cotton fiber quality and yield-related QTLs were identified in this CSSL population. Liang et al [39] detected 20 yield-related QTLs in BC 5 F 2 of this CSSL population.…”

“…In the present study, based on the phenotypic performance of this CSSL population [38], as well as previous findings from multi-ecological environment investigations [38–41], four introgression lines MBI9804, MBI9855, MBI9752, and MBI9134 with excellent fiber quality were selected as parental lines, and a double-crossed population of (MBI9804×MBI9855)×(MBI9752×MBI9134) was constructed. The introgressed Hai1 segments were evaluated in the segregating generations F 1 and F 2 and verified in the following F 2:3 generation using SSR markers.…”