Constitutive Overexpression of the OsNAS Gene Family Reveals Single-Gene Strategies for Effective Iron- and Zinc-Biofortification of Rice Endosperm

Johnson, Alexander; Kyriacou, Bianca; Callahan, Damien L.; Carruthers, Lorraine; Stangoulis, James; Lombi, Enzo; Tester, Mark

doi:10.1371/journal.pone.0024476

Cited by 338 publications

(268 citation statements)

References 37 publications

Supporting

Mentioning

250

Contrasting

Unclassified

Order By: Relevance

“…NA promotes high Fe content and Fe bioavailability in cereal grains (Lee et al, 2009;Zheng et al, 2010). NA is produced by NA synthase (NAS) (Shojima et al, 1989(Shojima et al, , 1990Herbik et al, 1999;Higuchi et al, 1999), and NAS genes have been recognized as valuable targets in the generation of micronutrient-enriched crops (Douchkov et al, 2005;Cassin et al, 2009;Lee et al, 2009;Wirth et al, 2009;Zheng et al, 2010;Johnson et al, 2011). Indeed, alterations of NAS gene activities affect NA contents, resulting in phenotypical changes related to uptake and distribution of Fe (Scholz et al, 1992;Douchkov et al, 2001Douchkov et al, , 2005Takahashi et al, 2003;) as well as of Zn (Deinlein et al, 2012;Haydon et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

The metal chelator nicotianamine promotes the bioavailability of Fe and reduces cellular Fe toxicity. For breeding Fe-efficient crops, we need to explore the fundamental impact of nicotianamine on plant development and physiology. The quadruple nas4x-2 mutant of Arabidopsis thaliana cannot synthesize any nicotianamine, shows strong leaf chlorosis, and is sterile. To date, these phenotypes have not been fully explained. Here, we show that sink organs of this mutant were Fe deficient, while aged leaves were Fe sufficient. Upper organs were also Zn deficient. We demonstrate that transport of Fe to aged leaves relied on citrate, which partially complemented the loss of nicotianamine. In the absence of nicotianamine, Fe accumulated in the phloem. Our results show that rather than enabling the long-distance movement of Fe in the phloem (as is the case for Zn), nicotianamine facilitates the transport of Fe from the phloem to sink organs. We delimit nicotianamine function in plant reproductive biology and demonstrate that nicotianamine acts in pollen development in anthers and pollen tube passage in the carpels. Since Fe and Zn both enhance pollen germination, a lack of either metal may contribute to the reproductive defect. Our study sheds light on the physiological functions of nicotianamine.

show abstract

Section: Introductionmentioning

confidence: 99%

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The Fe and Zn are mostly located in aleurone layer, but the percentage of Zn in polished rice (endosperm only) varies from around 75-84 % of the total Zn content including the aleurone layer. The case for Fe is only around 19-30% (Johnson et al 2011). Assuming 75% of the Zn is located in endosperm, IR10M195 (IR84842-35-3-1-1-2-2) is predicted to have Zn content in polished grain of around 26.76 ppm which is within the range of the targeted levels.…”

Section: Discussionmentioning

confidence: 95%

Yield and Zn content of biofortified rice genotypes in an Indonesian rice agro-ecosystem

Susanto¹,

Barokah²,

Hidayatullah³

et al. 2017

Nusantara Biosci

View full text Add to dashboard Cite

Abstract. Susanto U, Barokah U, Hidayatullah A, Satoto, Swamy M. 2017. Yield and Zn content of biofortified rice genotypes in an Indonesian rice . Approximately one-third of the world's population suffer from Zn deficiency causing significant socio-economic losses as a result of stunting and compromised immune system function. One strategy to overcome the problem is by developing rice cultivars with high grain Zn content (Zn Rice) to improve dietary intake.This study reports the yield and Zn content of 22 rice genotypes developed at the International Rice Research Institute (IRRI) and the Indonesian Centre for Rice Research (ICRR).The experiment was conducted in the Cirebon district of West Java province during the 2013 wet season (2013WS). Yield and grain Zn content (using an XRF machine) were measured. Five lines demonstrated higher yield (ranging from 7.0 to 8.9 t/ha) than the check variety Ciherang (5.2 t/ha), but had similar grain Zn content to the check variety Ciherang (23.4 ppm), ranging from 19.0 to 24.8 ppm. On the other hand, seven lines had higher grain Zn content (ranging from 30.0 to 34.2 ppm) compared to Ciherang, and five of these lines had comparable yield to Ciherang. The selected lines had acceptable agronomic traits, and are suitable for further testing and utilization, in addition to providing a foundation for future improvement in the dual goals of increasing the yield and nutritional value of rice.

show abstract

“…Here, multi-biofortification of rice is understood as rice enriched with folate, beta-carotene (provitamin A), zinc and iron, in line with De Steur et al 26 . Although iron 98,99 and/or zinc 100,101 may also be increased though transgenic approaches, and about 43 genes of five protein families are expected to be involved in rice 102 , they are not included in this study due to the lack of socio-economic studies on these GM biofortified rice crops. provitamin A in rice, the post-harvest losses (e.g.…”

Section: Gm Rice Crop With Health Benefits: the Case Of Rice Biofmentioning

confidence: 99%

Genetically Modified Rice with Health Benefits as a Means to Reduce Micronutrient Malnutrition

Steur¹,

Mogendi²,

Blancquaert³

et al. 2014

Wheat and Rice in Disease Prevention and Health

View full text Add to dashboard Cite

SummaryMicronutrient malnutrition, characterized by insufficient intake levels of vitamins and minerals, is a major public health problem that affects about 2 billion people worldwide. In order to reduce the burden of this 'hidden hunger', biofortification is more and more advocated as an alternative to the current micronutrient interventions. Through the enhancement of the micronutrient level of staple crops, it could address micronutrient malnutrition where the need is highest. Because staple crops are characterized by low micronutrient concentrations, genetic breeding techniques are often applied to increase specific vitamin levels, such as folate and pro-vitamin A. This study sheds a light on the global status of micronutrient malnutrition, biofortification and GM biofortified rice as both a GM food product with health benefits and a micronutrient intervention. Thereby, key consumer preference studies and cost-effectiveness analyses on Folate Biofortified Rice and Golden Rice are presented. Support is found for GM biofortified rice as a well-accepted GM food crop and a highly cost-effective health intervention.

show abstract

Constitutive Overexpression of the OsNAS Gene Family Reveals Single-Gene Strategies for Effective Iron- and Zinc-Biofortification of Rice Endosperm

Cited by 338 publications

References 37 publications

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

Yield and Zn content of biofortified rice genotypes in an Indonesian rice agro-ecosystem

Genetically Modified Rice with Health Benefits as a Means to Reduce Micronutrient Malnutrition

Contact Info

Product

Resources

About