Constitutive expression of the sunflower and chrysanthemum genes of the AP1/FUL group changes flowering timing in transgenic tobacco plants

Головешкина, Е. Н.; Shulga, O. A.; Щенникова, А. В.; Kamionskaya, A. M.; Skryabin, K. G.

doi:10.1134/s001249661005008x

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…Similar results were described for overexpression of these genes in transgenic tobacco (Goloveshkina et al 2010), for AP1 overexpression in tomato (Ellul et al 2004), and for NtMADS11 in tobacco (Jang et al 2002). Formation of a complex terminal flower by AP1 overexpression seems to be phenomenon specific only to Arabidopsis.…”

Section: Resultssupporting

confidence: 82%

“…unpublished data), and the same effect was observed for CDM111, HAM75, and HAM92 overexpression in Nicotiana tabacum (Goloveshkina et al 2010). Based on this information, transgenic chrysanthemum plants were generated constitutively overexpressing the HAM75, HAM92, and CDM111 cDNAs, and the resulting lines were analyzed for vegetative and reproductive growth.…”

Section: Resultssupporting

confidence: 79%

“…Strong expression of transgenes was detected in lines HAM75-13, HAM92-2, HAM92-31, and CDM111-16, whereas another five analyzed transgenic lines showed lower expression levels. A direct proportional correlation between the expression level of each gene and the flowering time of the corresponding plants observed in this study was similarly reported in HAM75 overexpressing tobacco plants (Goloveshkina et al 2010). For example, lines HAM92-2 and HAM92-31 developed flowers a few days earlier than line HAM92-6 with reduced HAM92 transcription; line CDM111-16 flowered on average 3 d earlier than CDM111-10, and line HAM75-13 initiated flower buds about 3 d earlier than HAM75-22 (Fig.…”

Section: Resultssupporting

confidence: 78%

“…Formation of a complex terminal flower by AP1 overexpression seems to be phenomenon specific only to Arabidopsis. At the same time, ectopic expression of AP1, or its orthologs, often causes a shortening of stem height and reduction in the number of leaves produced (Jang et al 2002;Goloveshkina et al 2010). Similar morphological changes were also shown for MdMADS2 (a FUL-like gene) when overexpressed in chrysanthemum (Han et al 2009).…”

Section: Resultsmentioning

confidence: 69%

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Overexpression of AP1-like genes from Asteraceae induces early-flowering in transgenic Chrysanthemum plants

Shulga

Mitiouchkina

Щенникова

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Chrysanthemum is one of the most important commercial cut flowers in the world. Early-flowering cultivars are required to produce quality chrysanthemum flowers with a lower cost of production. To shorten the vegetative growth phase of chrysanthemum, three AP1-like genes from Asteraceae were constitutively overexpressed in 80 independent transgenic chrysanthemum lines. All lines were characterized by PCR and RT-PCR and demonstrated that overexpression of compositae AP1-homologs in transgenic chrysanthemum under long-day conditions had no effect on plant development compared to non-transgenic controls. Conversely, under short-day conditions, transgenic plants commenced bud initiation 2 wk earlier than non-transgenic chrysanthemum plants. Subsequently, transgenic chrysanthemum flowers showed color earlier and resulted in full opening of inflorescences 3 wk prior to non-transgenic control plants. These results open new possibilities for genetic improvement and breeding of chrysanthemum cultivars.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 79%

Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 69%

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Overexpression of AP1-like genes from Asteraceae induces early-flowering in transgenic Chrysanthemum plants

Shulga

Mitiouchkina

Щенникова

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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“…LFY is thought to act as a signaling gateway, integrating signals from global floral pathway processes and activating downstream ABC genes that specify unique floral meristem and organ identities 18-23 . For example, APETALA1 (AP1), which determines floral meristem and organ identities in Arabidopsis, is directly activated by LFY [24][25][26][27] .LFY homologs have been identified among distantly related species [28][29][30][31][32][33][34][35] . LFY proteins from most species share conserved regions, such as a proline-rich region, a leucine zipper, an acidic region, and a basic region formed by identity and promote flowering time and cell proliferation 15,32,66,[72][73][74][75] .…”

mentioning

confidence: 99%

AfLFY, a LEAFY homolog in Argyranthemum frutescens, controls flowering time and leaf development

2020

Sci Rep

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Flowering is important for plant propagation and survival, and it is also closely related to human life. Identifying the molecular mechanisms underlying flower development is essential for plant improvement and breeding. Flower development is a complex physiological process that is regulated by multiple genes. LFY genes play important roles in the floral meristem transition and act as crucial integrators in regulating the floral gene network. Argyranthemum frutescens is an ornamental species cultivated for floral displays, yet little is known about molecular mechanisms driving its flower development. In this study, the LEAFY gene homologue, AfLFY, was identified and cloned from A. frutescens, and its role and expression patterns were characterized. Two distinct copies of AfLFY were found in the A. frutescens genome and both sequences contained a 1248 bp open reading frame that encoded 415 amino acids. The putative protein sequences have a typical LFY family domain. In addition, AfLFY was expressed at the highest levels in young leaves of the vegetative stage and in the shoot apical bud meristem of the reproductive stage. Phylogenetic analysis showed that AfLFY was most closely related to DFL from Chrysanthemum lavandulifolium. Subcellular localization studies revealed that AfLFY localized to the nucleus. Heterologous expression of AfLFY in transgenic tobacco plants shortened its period of vegetative growth, converted the lateral meristems into terminal flowers and promoted precocious flowering. In addition, transgenic plants exhibited obvious morphological changes in leaf shape. qRT-PCR analysis indicated that the expression levels genes related to flowering, FT, SOC1, and AP1 were significantly upregulated in AfLFY transgenic plants. Our findings suggested that the AfLFY gene plays a vital role in promoting flowering and leaf development in A. frutescens. These results laid a foundation for us to understand the mechanism of AfLFY in regulation flowering, and the results will be helpful in improving A. frutescens through molecular breeding.Flowering is a vital component of the plant life cycle that influences the success of plant reproduction. The transition from vegetative to reproductive development occurs in response to various endogenous and exogenous cues, and these cues are integrated by a complex molecular network 1-3 . Many floral integrator genes are involved in this molecular network, such as FLOWERING LOCUST (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), which work with the floral meristem identity gene LEAFY (LFY), to initiate the growth of floral meristems 4-7 . CONSTANS (CO) mediates the floral process in the photoperiod pathway and activates LFY expression directly or indirectly through other floral integrators [8][9][10] .LFY genes were initially described as floral meristem identity genes in Antirrhinum and Arabidopsis 11,12 , and were later shown to act as genetic switches directing the transition from inflorescence meristems to flower meristems 13 . Overexpression of LFY homologs s...

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Isolation and Characterization of an APETALA1-Like Gene from Pear (Pyrus pyrifolia)

Liu

Kong

et al. 2012

Plant Mol Biol Rep

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Constitutive expression of the sunflower and chrysanthemum genes of the AP1/FUL group changes flowering timing in transgenic tobacco plants

Cited by 9 publications

References 13 publications

Overexpression of AP1-like genes from Asteraceae induces early-flowering in transgenic Chrysanthemum plants

Overexpression of AP1-like genes from Asteraceae induces early-flowering in transgenic Chrysanthemum plants

AfLFY, a LEAFY homolog in Argyranthemum frutescens, controls flowering time and leaf development

Isolation and Characterization of an APETALA1-Like Gene from Pear (Pyrus pyrifolia)

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