Conservative system for dosage-dependent modulation of translational fidelity in eukaryotes

SFP1‐mediated prion‐dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1

“…It is known that overproduction of Sup35 or only its PrD is toxic to [ PSI + ] strains (Chernoff et al . ). Likewise, the presence of SUP35NM‐GFP (↑Sup35NM‐GFP) regulated by the CUP1 promoter led to growth inhibition of diploids even without copper addition (Fig.…”

“…The phenomenon of the [ PSI + ] prion toxicity had been described before the prion nature of [ PSI + ] was even proposed (Dagkesamanskaya & Ter‐Avanesyan ; Chernoff et al . ). The inability of the [ PRION + ] strain to retain the normal growth when the corresponding prion protein is overproduced is not unique to [ PSI + ]; for example, similarly, Rnq1 overproduction is toxic to [ PIN + ] strains (Douglas et al .…”

SFP1‐mediated prion‐dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1

“…It is known that overproduction of Sup35 or only its PrD is toxic to [ PSI + ] strains (Chernoff et al . ). Likewise, the presence of SUP35NM‐GFP (↑Sup35NM‐GFP) regulated by the CUP1 promoter led to growth inhibition of diploids even without copper addition (Fig.…”

“…The phenomenon of the [ PSI + ] prion toxicity had been described before the prion nature of [ PSI + ] was even proposed (Dagkesamanskaya & Ter‐Avanesyan ; Chernoff et al . ). The inability of the [ PRION + ] strain to retain the normal growth when the corresponding prion protein is overproduced is not unique to [ PSI + ]; for example, similarly, Rnq1 overproduction is toxic to [ PIN + ] strains (Douglas et al .…”

Evolutionary conservation of prion‐forming abilities of the yeast Sup35 protein

“…The Sup35NM region of P. methanolica is highly diverged from that of S. cerevisiae (Kushnirov et al ., 1990; Chernoff et al ., 1992a; see Fig. 2).…”

The three faces of Sup35

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993).…”

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993). In mammals, the nonessential N-terminal region of mammalian eRF3 has been shown to interact with PABP, the poly(A)-binding protein that is required for translation initiation and mRNA stabilization (Hoshino, Imai, Kobayashi, Uchida, & Katada, 1999).…”

SFP1‐mediated prion‐dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1

“…It is known that overproduction of Sup35 or only its PrD is toxic to [ PSI + ] strains (Chernoff et al . ). Likewise, the presence of SUP35NM‐GFP (↑Sup35NM‐GFP) regulated by the CUP1 promoter led to growth inhibition of diploids even without copper addition (Fig.…”

“…The phenomenon of the [ PSI + ] prion toxicity had been described before the prion nature of [ PSI + ] was even proposed (Dagkesamanskaya & Ter‐Avanesyan ; Chernoff et al . ). The inability of the [ PRION + ] strain to retain the normal growth when the corresponding prion protein is overproduced is not unique to [ PSI + ]; for example, similarly, Rnq1 overproduction is toxic to [ PIN + ] strains (Douglas et al .…”

Evolutionary conservation of prion‐forming abilities of the yeast Sup35 protein

“…The Sup35NM region of P. methanolica is highly diverged from that of S. cerevisiae (Kushnirov et al ., 1990; Chernoff et al ., 1992a; see Fig. 2).…”

The three faces of Sup35

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993).…”

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993). In mammals, the nonessential N-terminal region of mammalian eRF3 has been shown to interact with PABP, the poly(A)-binding protein that is required for translation initiation and mRNA stabilization (Hoshino, Imai, Kobayashi, Uchida, & Katada, 1999).…”

SFP1‐mediated prion‐dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1

“…It is known that overproduction of Sup35 or only its PrD is toxic to [ PSI + ] strains (Chernoff et al . ). Likewise, the presence of SUP35NM‐GFP (↑Sup35NM‐GFP) regulated by the CUP1 promoter led to growth inhibition of diploids even without copper addition (Fig.…”

“…The phenomenon of the [ PSI + ] prion toxicity had been described before the prion nature of [ PSI + ] was even proposed (Dagkesamanskaya & Ter‐Avanesyan ; Chernoff et al . ). The inability of the [ PRION + ] strain to retain the normal growth when the corresponding prion protein is overproduced is not unique to [ PSI + ]; for example, similarly, Rnq1 overproduction is toxic to [ PIN + ] strains (Douglas et al .…”

Evolutionary conservation of prion‐forming abilities of the yeast Sup35 protein

“…The Sup35NM region of P. methanolica is highly diverged from that of S. cerevisiae (Kushnirov et al ., 1990; Chernoff et al ., 1992a; see Fig. 2).…”

The three faces of Sup35

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993).…”

“…The C-terminus of Sup35p and other eRF3 proteins are important for translational termination function and are highly conserved (Chernoff et al, 1992;Kushnirov et al, 1988). However, regions outside of this C-terminal domain are dispensable for translational termination and are quite diverged (Chernoff et al, 1992;Ter-Avanesyan et al, 1993). In mammals, the nonessential N-terminal region of mammalian eRF3 has been shown to interact with PABP, the poly(A)-binding protein that is required for translation initiation and mRNA stabilization (Hoshino, Imai, Kobayashi, Uchida, & Katada, 1999).…”