Conservation of a novel protein associated with an antibiotic efflux operon in Burkholderia cenocepacia

Nair, Bindu M.; Joachimiak, Łukasz A.; Chattopadhyay, Sujay; Montano, Idalia; Burns, Jane L.

doi:10.1016/j.femsle.2005.03.027

Cited by 13 publications

(13 citation statements)

References 47 publications

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“…ZPS has been extensively tested with different genes from Escherichia coli of diverse origin [6,9,14,15], Burkholderia cenocepacia [16], Vibrio vulnificus and hepatitis C virus genotype 1 [unpublished data].…”

Section: Resultsmentioning

confidence: 99%

ZPS: visualization of recent adaptive evolution of proteins

2007

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Background: Detection of adaptive amino acid changes in proteins under recent short-term selection is of great interest for researchers studying microevolutionary processes in microbial pathogens or any other biological species. However, independent occurrence of such point mutations within genetically diverse haplotypes makes it difficult to detect the selection footprint by using traditional molecular evolutionary analyses. The recently developed Zonal Phylogeny (ZP) has been shown to be a useful analytic tool for identifying the footprints of short-term positive selection. ZP separates protein-encoding genes into evolutionarily long-term (with silent diversity) and short-term (without silent diversity) categories, or zones, followed by statistical analysis to detect signs of positive selection in the short-term zone. However, successful broad application of ZP for analysis of large haplotype datasets requires automation of the relatively labor-intensive computational process.

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Section: Resultsmentioning

confidence: 99%

ZPS: visualization of recent adaptive evolution of proteins

2007

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“…This operon has been identified in B. cenocepacia , and the llpE gene is without parallel in previously reported efflux operons. The llpE gene was shown to be the most prevalent in B. cenocepacia , with a high degree of sequence conservation [7, 8]. The lack of this B. cenocepacia marker in genomes of ST858 and ST859 is particularly interesting in view of the unusual/unique phylogenetic status of these strains, as the results of evolutionary distances between concatenated nucleotide sequences of seven alleles that grouped the two STs close to B. multivorans were not in concordance with MLST scheme which identified them as B. cenocepacia [13].…”

Section: Discussionmentioning

confidence: 99%

“…The first gene in the bpeEF-oprC operon, llpE , was shown to be the most prevalent in B . cenocepacia , and is co-transcribed with the genes encoding the BpeEF-OprC efflux pump components that was shown to be of importance for resistance to antibiotics [7, 8]. Additionally, the O-antigen portion (coded by the wbiI gene) of the LPS molecule is important for resistance of Bcc to serum-mediated killing [9].…”

Section: Introductionmentioning

confidence: 99%

Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients

Malešević

Vasiljević

Sovtić

et al. 2017

Antimicrob Resist Infect Control

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Background Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32).MethodsBiofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed.ResultsBiofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene.ConclusionVariations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.

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“…40,43 As a result of multiple resistance mechanisms, including an efflux pump, chronic infection with BCC is associated with an 80% prevalence of MDR, defined as resistance to 3 or more classes of antibiotics. 40,44 In this setting, some experts recommend synergy testing to determine optimal antibiotic combinations, although its efficacy is uncertain. 45 There are limited data about the optimal treatment approach after transplant, although most centers recommend prolonged combination antibiotic therapy given the high risk of fatal infection after transplant.…”

Section: Specific Organismsmentioning

confidence: 99%