Connexin-43 is a promising target for lycopene preventing phthalate-induced spermatogenic disorders

Wang, Tianhao; Zhao, Bi-Chen; Li, Jinyang; Talukder, Milton; Li, Jinlong

doi:10.1016/j.jare.2022.09.001

Cited by 13 publications

(7 citation statements)

References 53 publications

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“…The permeability of the BTB in vitro was detected by TER, as described previously [ 65 ]. Briefly, SCs (1 × 10 6 cells/cm 2 ) were seeded in a transwell chamber (Cat# PTHT24H48, Millipore, Bedford, TX, USA) and cultured to allow BTB assembly.…”

Section: Methodsmentioning

confidence: 99%

Zearalenone Induces Blood-Testis Barrier Damage through Endoplasmic Reticulum Stress-Mediated Paraptosis of Sertoli Cells in Goats

Liu,

et al. 2023

IJMS

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Zearalenone (ZEA) is present worldwide as a serious contaminant of food and feed and causes male reproductive toxicity. The implication of paraptosis, which is a nonclassical paradigm of cell death, is unclear in ZEA-induced male reproductive disorders. In this study, the toxic effects of ZEA on the blood-testis barrier (BTB) and the related mechanisms of paraptosis were detected in goats. ZEA exposure, in vivo, caused a significant decrease in spermatozoon quality, the destruction of seminiferous tubules, and damage to the BTB integrity. Furthermore, ZEA exposure to Sertoli cells (SCs) in vitro showed similar dysfunction in structure and barrier function. Importantly, the formation of massive cytoplasmic vacuoles in ZEA-treated SCs corresponded to the highly swollen and dilative endoplasmic reticulum (ER), and paraptosis inhibition significantly alleviated ZEA-induced SC death and vacuolization, which indicated the important contribution of paraptosis in ZEA-induced BTB damage. Meanwhile, the expression of ER stress marker proteins was increased after ZEA treatment but decreased under the inhibition of paraptosis. The vacuole formation and SC death, induced by ZEA, were remarkably blocked by ER stress inhibition. In conclusion, these results facilitate the exploration of the mechanisms of the SC paraptosis involved in ZEA-induced BTB damage in goats.

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Section: Methodsmentioning

confidence: 99%

Zearalenone Induces Blood-Testis Barrier Damage through Endoplasmic Reticulum Stress-Mediated Paraptosis of Sertoli Cells in Goats

Liu,

et al. 2023

IJMS

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“…The BTB is formed between Sertoli cells, where it creates a unique microenvironment for spermatogenesis 46 . If BTB is disrupted, spermatogenesis can be severely impaired, and germ cells will loss due to cell death 47 , which is similar to the phenotype in SARS-CoV-2 GFP/ΔN infected AMH-hACE2-N F/F testes.…”

Section: Sars-cov-2 Infection Disrupts the Integrity Of Btbmentioning

confidence: 99%

A tissue specific-infection mouse model of SARS-CoV-2

Yang

Liu

et al. 2023

Cell Discov

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Animal models play crucial roles in the rapid development of vaccines/drugs for the prevention and therapy of COVID-19, but current models have some deficits when studying the pathogenesis of SARS-CoV-2 on some special tissues or organs. Here, we generated a human ACE2 and SARS-CoV-2 NF/F knockin mouse line that constitutively expresses human ACE2 and specifically expresses SARS-CoV-2 N gene induced by Cre-recombinase. By crossing with Cre transgenic lines allowing for lung-specific and constitutive expression, we generated lung-specific (Sftpc-hACE2-NF/F) and constitutive SARS-CoV-2 N (EIIa-hACE2-NF/F) expressing mice. Upon intranasal infection with a SARS-CoV-2 GFP/ΔN strain which can only replicate in SARS-CoV-2 N expressed cells, we demonstrated that both the Sftpc-hACE2-NF/F and EIIa-hACE2-NF/F mice support viral replication. Consistent with our design, viral replication was limited to the lung tissues in Sftpc-hACE2-NF/F mice, while the EIIa-hACE2-NF/F mice developed infections in multiple tissues. Furthermore, our model supports different SARS-CoV-2 variants infection, and it can be successfully used to evaluate the effects of therapeutic monoclonal antibodies (Ab1F11) and antiviral drugs (Molnupiravir). Finally, to test the effect of SARS-CoV-2 infection on male reproduction, we generated Sertoli cell-specific SARS-CoV-2 N expressed mice by crossing with AMH-Cre transgenic line. We found that SARS-CoV-2 GFP/ΔN strain could infect Sertoli cells, led to spermatogenic defects due to the destruction of blood-testis barrier. Overall, combining with different tissue-specific Cre transgenic lines, the human ACE2 and SARS-CoV-2 NF/F line enables us to evaluate antivirals in vivo and study the pathogenesis of SARS-CoV-2 on some special tissues or organs.

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“… 50 In related studies, we found that spermatogonia have great significance in the study of male infertility diseases, 51 , 52 especially when examining sperm quality, and the number of spermatogonia largely affects the quality of sperm 53 , 54 and testicular development processes. 55 However, the current relevant research is more limited, and the mechanism has not yet been elucidated. Whether sperm cells can be formed for normal fertilization after long-term in vitro culture is a current concern.…”

Section: Exosomes and Testicular Developmentmentioning

confidence: 99%

The emerging role of exosomes in the development of testicular

Liu

Dai

et al. 2023

Asian Journal of Andrology

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The mechanisms of testicular development in mammals are complex. Testis is an organ that produces sperm and secretes androgens. It is rich in exosomes and cytokines that mediate signal transduction between tubule germ cells and distal cells, promoting testicular development and spermatogenesis. Exosomes are nanoscale extracellular vesicles that transmit information between cells. By transmitting information, exosomes play an important role in male infertility diseases such as azoospermia, varicocele, and testicular torsion. However, due to the wide range of sources of exosomes, extraction methods are numerous and complex. Therefore, there are many difficulties in studying the mechanisms of exosomal effects on normal development and male infertility. Therefore, in this review, first, we introduce the formation of exosomes and methods for culturing testis and sperm. Then, we introduce the effects of exosomes on different stages of testicular development. Finally, we summarize the prospects and shortcomings of exosomes when used in clinical applications. We lay the theoretical foundation for the mechanism of the influence of exosomes on normal development and male infertility.

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Connexin-43 is a promising target for lycopene preventing phthalate-induced spermatogenic disorders

Cited by 13 publications

References 53 publications

Zearalenone Induces Blood-Testis Barrier Damage through Endoplasmic Reticulum Stress-Mediated Paraptosis of Sertoli Cells in Goats

Zearalenone Induces Blood-Testis Barrier Damage through Endoplasmic Reticulum Stress-Mediated Paraptosis of Sertoli Cells in Goats

A tissue specific-infection mouse model of SARS-CoV-2

The emerging role of exosomes in the development of testicular

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