Conjugating a groove-binding motif to an Ir(<scp>iii</scp>) complex for the enhancement of G-quadruplex probe behavior

Wang, Wanhe; Mao, Zhifeng; Kang, Tian-Shu; Wong, Chun‐Yuen; Mergny, Jean‐Louis; Leung, Chung‐Hang; Ma, Dik‐Lung

doi:10.1039/c6sc00001k

Cited by 152 publications

(55 citation statements)

References 48 publications

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“…Given that nature is capable of synthesizing ad iverse range of highly functionalized and complex molecular architectures, starting from al imited set of buildingb locks researchers could obtain inspiration from naturally derived scaffolds [61] for future modification of organic [62] or inorganic compounds [63] for the development of as ensing strategy based on G-quadruplex-selective probes. Considering their metabolic complexity,d ynamic variability,a nd individual differences in living organisms, mutual verificationf or both in vitro and in vivo assays is highly suggestedf or better understanding or even controlling of substance metabolism processes.…”

Section: Discussionmentioning

confidence: 99%

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Dong

et al. 2017

Chemistry An Asian Journal

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G-Quadruplexes can be induced to form guanine-rich DNA sequences by certain small molecules or metal ions. In concert with an appropriate signal transducer, such as a fluorescent dye or a phosphorescent metal complex, the ligand-recognition event can be transduced into a luminescent response. This focus review aims to highlight recent examples of aptamer-based and metal-mediated G-quadruplex assays for the detection of small molecules and toxic substances in the last three years. We discuss the mechanisms and features of the different assays and present an outlook and a perspective for the future of this field.

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Section: Discussionmentioning

confidence: 99%

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Dong

et al. 2017

Chemistry An Asian Journal

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“…Compared to other sensing modes, luminescence detection offers the advantage of easy visualization, simple operation, high sensitivity, portability, and robustness [15]. In this context, phosphorescent transition metal complexes have emerged as attractive scaffolds for the development of chemosensors owing to their desirable properties such as long lifetime, large Stokes' shift, high stability, tunable excitation and emission wavelengths, and capability for achieving high selectivity of biological molecules [72,73].…”

Section: Discussion and Outlookmentioning

confidence: 99%

“…However, these methods tend to be tedious and time-consuming process, and may require specialist instrumentation and/or expertise. Recently, optical strategies for real-time and in-field sensing of biomarkers have been explored, including methods based on green fluorescent protein [14], DNA sequence [15][16][17][18], nanoparticles [19][20][21], quantum dots [22][23][24], organic dyes [25,26] and metal complexes [27]. Fluorescent organic dyes, in particular, have received intense interest for biomarker detection.…”

Section: Introductionmentioning

confidence: 99%

Group 8–9 Metal-Based Luminescent Chemosensors for Protein Biomarker Detection

et al. 2018

J. Anal. Test.

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The development of transition metal complex-based luminescent chemosensors has recently aroused increasing interest for protein biomarker labelling and detection, especially for the real-time diagnosis and treatment of disease. This is owing to their unique photophysical properties, particularly their long-lived and environmentally sensitive emission, which can be easily controlled via the structural modification of ligands. In this overview, we highlight recent examples of protein biomarker detection using group 8-9 metal-based luminescent chemosensors, including the frequently employed ruthenium(II) and iridium(III) complexes. Various mechanisms and sensing modes are described and compared, and the outlook and future directions of this field are discussed as well.

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“…The formation of a ternary complex of the target RNA, a circular DNA template, and a DNA primer initiates RCA, results in a G4 string that contains multiple repeats of a 27-mer sequence (27Myc) forming a parallel G4, [32][33][34][35][36][37] which can be found in the nuclease hypersensitivity element region of the c-Myc P1 promoter. Because the thioflavin T derivative ThT-HE preferably binds to 27Myc and strongly emits fluorescence, we anticipated that the G4 strings produced would be specifically stained by ThT-HE with excellent contrast, even with coexistent RNAs and DNAs forming canonical structures.…”

Section: Introductionmentioning

confidence: 99%

Novel One-Tube-One-Step Real-Time Methodology for Rapid Transcriptomic Biomarker Detection: Signal Amplification by Ternary Initiation Complexes

et al. 2016

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We have developed a novel RNA detection method, termed signal amplification by ternary initiation complexes (SATIC), in which an analyte sample is simply mixed with the relevant reagents and allowed to stand for a short time under isothermal conditions (37 °C). The advantage of the technique is that there is no requirement for (i) heat annealing, (ii) thermal cycling during the reaction, (iii) a reverse transcription step, or (iv) enzymatic or mechanical fragmentation of the target RNA. SATIC involves the formation of a ternary initiation complex between the target RNA, a circular DNA template, and a DNA primer, followed by rolling circle amplification (RCA) to generate multiple copies of G-quadruplex (G4) on a long DNA strand like beads on a string. The G4s can be specifically fluorescence-stained with N(3)-hydroxyethyl thioflavin T (ThT-HE), which emits weakly with single- and double-stranded RNA/DNA but strongly with parallel G4s. An improved dual SATIC system, which involves the formation of two different ternary initiation complexes in the RCA process, exhibited a wide quantitative detection range of 1-5000 pM. Furthermore, this enabled visual observation-based RNA detection, which is more rapid and convenient than conventional isothermal methods, such as reverse transcription-loop-mediated isothermal amplification, signal mediated amplification of RNA technology, and RNA-primed rolling circle amplification. Thus, SATIC methodology may serve as an on-site and real-time measurement technique for transcriptomic biomarkers for various diseases.

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Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior

Abstract: G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a Ir(iii) complex to generate a highly selective DNA probe.

Cited by 152 publications

References 48 publications

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Group 8–9 Metal-Based Luminescent Chemosensors for Protein Biomarker Detection

Novel One-Tube-One-Step Real-Time Methodology for Rapid Transcriptomic Biomarker Detection: Signal Amplification by Ternary Initiation Complexes

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