Confocal-based cell-specific finite element modeling extended to study variable cell shapes and intracellular structures: The example of the adipocyte

Or-Tzadikario, Shira; Gefen, Amit

doi:10.1016/j.jbiomech.2010.09.012

Cited by 34 publications

(28 citation statements)

References 15 publications

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“…A 3D formulation also allows for the simulation of realistic polarised cell geometries and the inclusion of a cell nucleus. Cell compression has been used in a range of in vitro studies to quantify the mechanical properties of different cell types, including endothelial cells (Caille et al, 2002), fibroblasts Ott, JCS), myoblasts, Peeters 2005, adipocytes (Or-Tzadikario andGefen, 2011) and MSCs, osteoblasts, chondrocytes, adipocytes (Darling et al, 2008). Previous computational investigations have assumed axisymmetric geometries for compression (Caille et al, 2002;Nguyen et al, 2010;Ofek et al, 2009b) and micropipette aspiration (Haider and Guilak, 2002;Rowat et al, 2005) In the current study, the contractile cytoskeleton has been considered in a 3D, predictive framework with no predefined SF arrangement.…”

Section: Discussionmentioning

confidence: 99%

Numerical investigation of the active role of the actin cytoskeleton in the compression resistance of cells

Ronan

Deshpande

McMeeking

et al. 2012

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionmentioning

confidence: 99%

Numerical investigation of the active role of the actin cytoskeleton in the compression resistance of cells

Ronan

Deshpande

McMeeking

et al. 2012

Journal of the Mechanical Behavior of Biomedical Materials

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“…In our simulations of cell indentation, the endothelial cell is modeled as a nearly incompressible (14,27) hyperelastic neo-Hookean (13,28,29) material, with a Poisson's ratio of 0.49 and a Young's modulus of 1 kPa. The microindenter's spherical tip is considered infinitely rigid compared to the cell.…”

Section: Simulations Of Cell Indentationmentioning

confidence: 99%

Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Gonzalez‐Rodriguez

Guillou

Cornat

et al. 2016

Biophysical Journal

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We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter's tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microindenter's deflection. We show that plasma membrane rupture of endothelial cells occurs at a well-defined value of the applied compressive stress. As a point of reference, we use numerical simulations to estimate the magnitude of the compressive stresses exerted on endothelial cells during the deployment of a stent.

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“…The PM strains were evaluated by means of confocalmicroscopy-based three-dimensional (3D) cell-specific FE modeling. 33,38,40 The experimental and modeling procedures are described in detail below.…”

Section: Methodsmentioning

confidence: 99%

“…33,38,40 To allow for variability in PM strains to be estimated as well, we used 3D models of five different randomly selected C2C12 myoblasts. The cell-specific FE modeling methodology is described in detail in the aforementioned publications, and hence, here we review it briefly, for completeness.…”

Section: Simulations Of the Pm Permeability Studies To Evaluatementioning

confidence: 99%

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Relationship Between Strain Levels and Permeability of the Plasma Membrane in Statically Stretched Myoblasts

Slomka

Gefen

2011

Ann Biomed Eng

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Deep tissue injury (DTI) is a life-threatening type of pressure ulcer which initiates subdermally with muscle necrosis at weight-bearing anatomical locations, where localized elevated tissue strains exist. Though it has been suggested that excessive sustained soft tissue strains might compromise cell viability, which then initiates the DTI, there is no experimental evidence to describe how specifically such a process might take place. Here, we experimentally test the hypothesis that macroscopic tissue deformations translated to cell-level deformations and in particular, to localized tensile strains in the plasma membrane (PM) of cells, increase the permeability of the PM which could disrupt vital transport processes. In order to determine whether PM permeability changes can occur due to static stretching of cells we measured the uptake of fluorescein isothiocyanate (FITC)-labeled Dextran (molecular weight = 4 kDa) by deformed vs. undeformed myoblasts, using a fluorescence-activated cell sorting (FACS) method. These PM permeability changes were then correlated with tensile strains in the PM which correspond to the levels of substrate tensile strain (STS) that were applied in the experiments. The PM strains were evaluated by means of confocal-microscopy-based cell-specific finite element (FE) modeling. The FACS studies demonstrated a statistically significant rise in the uptake of the FITC-labeled Dextran with increasing STS levels in the STS ≤ 12% domain, which thereby indicates a rise in the permeability of the PM of the myoblasts with the extent of the applied cellular deformation. The cell-specific FE modeling simulating the experiments further demonstrated that applying average PM tensile strains which exceed 3%, or, applying peak PM tensile strains over 9%, substantially increases the permeability of the PM of myoblasts to the Dextran. Moreover, the permeability of the PM grew rapidly with any further increase in PM strains, though there were no significant changes in the uptake above average and peak PM tensile strain values of 9 and 26%, respectively. These results provide an experimental basis for studying the theory that cell-level deformation-diffusion relationships may be involved in determining the tolerance of soft tissues to sustained mechanical loading, as relevant to the etiology of DTI.

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Confocal-based cell-specific finite element modeling extended to study variable cell shapes and intracellular structures: The example of the adipocyte

Cited by 34 publications

References 15 publications

Numerical investigation of the active role of the actin cytoskeleton in the compression resistance of cells

Numerical investigation of the active role of the actin cytoskeleton in the compression resistance of cells

Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Relationship Between Strain Levels and Permeability of the Plasma Membrane in Statically Stretched Myoblasts

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