Confinement of Transmembrane Cell Receptors in Tunable Stripe Micropatterns

Abstract

We report a simple method to confine transmembrane cell receptors in stripe micropatterns of a lipid/lipopolymer monolayer, which are formed as result of the transfer onto a solid substrate. The stripes are aligned perpendicular to the meniscus, whose periodicity can systematically be tuned by the transfer velocity. This strongly suggests the dominant role of the cooperative interaction between the film and substrate. Selective fluorescence labeling of lipids and lipopolymers confirms that the observed patterns coincide with the demixing of two species. Covalent coupling of polymer headgroups enables us to use the stripe patterns as a support for a lipid bilayer membrane. Spreading of lipid vesicles with platelet integrin alphaIIbbeta3 on a self-assembled membrane micropattern demonstrates that cell adhesion receptors are selectively incorporated into the lipopolymer-rich region. The method established here provides us with a tunable template for the confinement of receptor proteins to geometrically control the cell adhesion.

Mechanism of Regular Pattern Formation in Reactive Dewetting

“…[1] These type of patterns have been recently observed in other monolayer systems [4] opening the possibility of extending LB lithography to other pattern-generating chemical systems. The patterns can be measured with scanning force microscopy (SFM) or optical microscopy, before or after etching the structures into silicon.…”

Modulation of Substrate–Membrane Interactions by Linear Poly(2‐methyl‐2‐oxazoline) Spacers Revealed by X‐ray Reflectivity and Ellipsometry

“…A high transfer speed of 30 mm min À1 was chosen to avoid demixing of the lipid/lipopolymer mixture. [35] Throughout this study, a transfer ratio of unity was guaranteed within the experimental error of AE 2 %.…”

Poly(2‐oxazoline): eine Polymerklasse mit vielfältigen Anwendungsmöglichkeiten

“…Während der Übertragung der gemischten Phospholipid/LipopolymerMonoschicht wurde die spontane Bildung eines Streifenmusters parallel zur Übertragungsrichtung beobachtet. [111] Die Verwendung fluoreszenzmarkierter Polymere offenbarte eine durch Alkylkettenkondensation angetriebene Trennung [115][116][117] und Montemagno [118][119][120] über Polymersome aus Poly(2-oxazolin)-block-poly(dimethylsiloxan)-block-poly(2-oxazolin)-Triblockcopolymeren als biomimetische Membranen erwähnt werden.…”