Conditions for isolation and culture of porcine myogenic satellite cells

Doumit, M. E.; Merkel, R. A.

doi:10.1016/0040-8166(92)90098-r

Cited by 93 publications

(78 citation statements)

References 17 publications

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“…Preplatening for 30 min on either primaria or uncoated plates, followed by retrieval of nonattached cells, e.g., myogenic satellite cells, was also not effective ( Fig. 1), which is in accordance with the findings of Doumit and Merkel (9). Spinning the cells for 20 min at 1,250 g through a density gradient of 35/50/70% Percoll did not improve the fusion percentage (Fig.…”

Section: Methodssupporting

confidence: 80%

Reactive oxygen species are important mediators of taurine release from skeletal muscle cells

Ørtenblad

Young

Oksbjerg

et al. 2003

American Journal of Physiology-Cell Physiology

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The present study illustrates elements of the signal cascades involved in the activation of taurine efflux pathways in myotubes derived from skeletal muscle cells. Exposing primary skeletal muscle cells, loaded with 14C-taurine, to 1) hypotonic media, 2) the phospholipase A2 (PLA2) activator melittin, 3) anoxia, or 4) lysophosphatidyl choline (LPC) causes an increase in 14C-taurine release and a concomitant production of reactive oxygen species (ROS). The antioxidants butulated hydroxy toluene and vitamin E inhibit the taurine efflux after cell swelling, anoxia, and addition of LPC. The muscle cells possess two separate taurine efflux pathways, i.e., a swelling- and melittin-induced pathway that requires 5-lipoxygenase activity for activation and a LPC-induced pathway. The two pathways are distinguished by their opposing sensitivity toward the anion channel blocker DIDS and cholesterol. These data provide evidence for PLA2 products and ROS as key mediators of the signal cascade leading to taurine efflux in muscle.

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Section: Methodssupporting

confidence: 80%

Reactive oxygen species are important mediators of taurine release from skeletal muscle cells

Ørtenblad

Young

Oksbjerg

et al. 2003

American Journal of Physiology-Cell Physiology

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“…Protocols have been developed to isolate satellite cells in bulk utilizing their characteristic proliferation and adhesion characteristics under defined culturing conditions (144,364,425). It is noteworthy that the satellite cell progeny cultured on regular collagen-coated plastic dishes and under activation conditions, also called satellite cell-derived primary myoblasts, are molecularly and functionally distinct from the satellite cells freshly isolated from muscles (160,177,350).…”

Section: B Identification and Isolation Of Satellite Cellsmentioning

confidence: 99%

Satellite Cells and the Muscle Stem Cell Niche

Yin

Price

Rudnicki

2013

Physiological Reviews

1,715

1,808

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LYin H, Price F, Rudnicki MA. Satellite Cells and the Muscle Stem Cell Niche. Physiol Rev 93: 23-67, 2013; doi:10.1152/physrev.00043.2011.-Adult skeletal muscle in mammals is a stable tissue under normal circumstances but has remarkable ability to repair after injury. Skeletal muscle regeneration is a highly orchestrated process involving the activation of various cellular and molecular responses. As skeletal muscle stem cells, satellite cells play an indispensible role in this process. The self-renewing proliferation of satellite cells not only maintains the stem cell population but also provides numerous myogenic cells, which proliferate, differentiate, fuse, and lead to new myofiber formation and reconstitution of a functional contractile apparatus. The complex behavior of satellite cells during skeletal muscle regeneration is tightly regulated through the dynamic interplay between intrinsic factors within satellite cells and extrinsic factors constituting the muscle stem cell niche/microenvironment. For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved our understanding of skeletal muscle biology. Here, we review some recent advances, with focuses on functions of satellite cells and their niche during the process of skeletal muscle regeneration.

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“…Satellite cells were isolated from the three bovine muscle depots (longissimus dorsi, deep pectoral and semitendinosus) as described by Doumit and Merkel (1992). Briefly, the muscles were excised, trimmed of visible connective tissue, and minced with fine sharp scissors.…”

Section: Cell Preparationmentioning

confidence: 99%

Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition

Rajesh¹,

Kim²,

Park³

et al. 2009

Journal of Animal Science and Technology

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The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.

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Conditions for isolation and culture of porcine myogenic satellite cells

Cited by 93 publications

References 17 publications

Reactive oxygen species are important mediators of taurine release from skeletal muscle cells

Reactive oxygen species are important mediators of taurine release from skeletal muscle cells

Satellite Cells and the Muscle Stem Cell Niche

Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition

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