a b s t r a c tA real matrix A = (a ij ) 1≤i,j,≤n is said to be almost strictly totally negative if it is almost strictly sign regular with signature ε = (−1, −1, . . . , −1), which is equivalent to the property that all its nontrivial minors are negative. In this paper an algorithmic characterization of nonsingular almost strictly totally negative matrices is presented.