Conditioned medium from <i>Bifidobacteria infantis</i> protects against <i>Cronobacter sakazakii</i>-induced intestinal inflammation in newborn mice

Weng, Meiqian; Ganguli, Kriston; Zhu, Weishu; Shi, Hai Ning; Walker, W. Allan

doi:10.1152/ajpgi.00183.2013

Cited by 45 publications

(38 citation statements)

References 42 publications

(48 reference statements)

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“…These experiments suggest that B. infantis produce exogenous substances that promote maturation of the immature innate immune response (68). This supernatant from B. infantis attenuates Cronobacter sakazakii- induced enteritis in a newborn mouse model ( C. sakazakii is a contaminant of powdered infant formulas associated with both sepsis and NEC in premature infants) (69). In a rat model of NEC, administration of B. infantis decreased expression of IL6, IL8, TNFα, IL23, and iNOS, decreased the expression of antimicrobial peptides, altered expression of intestinal mucus-related proteins, and decreased the incidence of NEC (17).…”

Section: Mechanisms Of Observed Protective Effectsmentioning

confidence: 99%

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

et al. 2014

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Oligosaccharides are abundant in human milk. Production of these highly diverse structures requires significant energy expenditure by the mother and yet these human milk oligosaccharides offer no direct nutritive value to her infant. A primary function of human milk oligosaccharides is to shape the infant’s intestinal microbiota with life-long consequences. Bifidobacterium longum subspecies infantis (B. infantis) is unique among gut bacteria in its prodigious capacity to digest and consume any human milk oligosaccharide structure, the result of a large repertoire of bacterial genes encoding an array of glycosidases and oligosaccharide transporters not found in other bacterial species. In vitro, B. infantis grows better than other bacterial strains in the presence of human milk oligosaccharides, displays anti-inflammatory activity in premature intestinal cells, and decreases intestinal permeability. In premature infants, B. infantis given in combination with human milk increases B. infantis and decreases Enterobacteriaceae in the feces. Probiotics containing B. infantis decrease the risk of necrotizing enterocolitis in premature infants. Colonization with B. infantis is also associated with increased vaccine responses. Probiotic organisms have historically been selected based on ease of production and stability. The advantages of B. infantis, selected through coevolution with human milk glycans, present an opportunity for focused manipulation of the infant intestinal microbiota.

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Section: Mechanisms Of Observed Protective Effectsmentioning

confidence: 99%

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

et al. 2014

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“…42 Culture medium from B. infantis decreases the severity of infection with C. sakazaki in the mouse with attenuation of three mechanisms of pathogenesis: induction of IL1β and TNFα, decrease in mucin production, and increase in apoptosis. 43 …”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Impact of probiotics on necrotizing enterocolitis

Underwood

2017

Seminars in Perinatology

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A large number of randomized placebo-controlled clinical trials and cohort studies have demonstrated a decrease in the incidence of necrotizing enterocolitis with administration of probiotic microbes. These studies have prompted many neonatologists to adopt routine prophylactic administration of probiotics while others await more definitive studies and/or probiotic products with demonstrated purity and stable numbers of live organisms. Cross-contamination and inadequate sample size limit the value of further traditional placebo-controlled randomized controlled trials. Key areas for future research include mechanisms of protection, optimum probiotic species or strains (or combinations thereof) and duration of treatment, interactions between diet and the administered probiotic, and the influence of genetic polymorphisms in the mother and infant on probiotic response. Next generation probiotics selected based on bacterial genetics rather than ease of production and large cluster-randomized clinical trials hold great promise for NEC prevention.

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“…In studies from this laboratory (73), we have identified a secreted product from the same B. infantis which on partial characterization is likely a glycan of less than 30 kD size. This factor has an anti-inflammatory effect in a fetal small intestinal cell line (H4) and functions by causing a maturation of the innate inflammatory immune response to an inflammatory stimulus with a reduction in the expression of TLR-4, its signaling molecules and its transcription factor NFκB genes and an increase in negative regulators of this response (TOLLIP, SIGIIR, and A-20) as seen in mature enterocytes (73,74). Finally, a recent study of the molecular response of intestinal bacteria to breast vs. infant formula feeding has shown that the products of breastfed intestinal bacteria have a greater effect on protective genes in enterocytes than products of formula-fed infants (75).…”

Section: Breastfeeding and Intestinal Colonizationmentioning

confidence: 99%

Breast milk, microbiota, and intestinal immune homeostasis

2014

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Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice

Abstract: Weng M, Ganguli K, Zhu W, Shi HN, Walker WA. Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice.

Cited by 45 publications

References 42 publications

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

Impact of probiotics on necrotizing enterocolitis

Breast milk, microbiota, and intestinal immune homeostasis

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