Concurrent Modulation of Competitive Mechanisms to Design Stimuli‐Responsive Ln‐MOFs: A Light‐Operated Dual‐Mode Assay for Oxidative DNA Damage

Abstract

The identification of biomolecules for disease diagnosis requires facile analytical technologies with high precision and reliability. Several signal transduction pathways have inspired the development of various bioanalytical systems. However, most systems are greatly limited by a single-mechanism/ mode assay, which easily results in false-positive/negative results. Herein, a multiple-mechanism-driven optical biosensor for 8-oxo-2′-deoxyguanosine (8-oxo-dG), an early pathological signature of DNA lesions and various diseases, is designed by assembling adenine as a recognition element, mellitic acid as energy donors and Eu 3+ as signal reporters into one metalorganic framework (MOF) system. Significantly, by regulating the delicate competition between the different mechanisms, the fabricated single platform (Eu-ade-MOF) concurrently provides two switchable approaches for rapid qualitative (30 s and 4 min) and quantitive (ppb level) recognition of 8-oxo-dG in both complex artificial and real human urine environments. Compared with those single-mechanism/mode-driven detections, this light-operated dual-mode analysis system can inherently boost the analysis reliability and largely minimize the chances of false negatives/positives for a non-invasive diagnosis of DNA damage and related diseases. This work represents the first effort in designing a luminescent sensor coupling multiple mechanisms in a single interface to determine DNA damage degree and provides a new approach for developing multimode analysis platforms for human health monitoring.

Multivalued Logic Assay of the Disease Marker of α-Ketoglutaric Acid by a Luminescent MOF-Based Biosensor

“…Then, the as-prepared MOF powder (2.5 mg) was dispersed into water (0.5 mL) under ultrasonication to form an aqueous suspension and subsequently added to the above agarose solution (2.5 mL) dropwise. After sonicating, the mixtures were cooled down sufficiently until a semi-solid hydrogel was formed …”

“…After sonicating, the mixtures were cooled down sufficiently until a semisolid hydrogel was formed. 37 Procedures for α-KA Detection. In the typical and selective assay for α-KA, aqueous solutions (5 × 10 −3 M, 10 mL) of α-KA and various biologically relevant species, including L-isoleucine (LLE), Lleucine (LEU), dopamine (DA), phenylglyoxal (PGO), L-threonine (THR), sodium pyruvate (PAS), L-proline (PRO), L-tryptophan (TRP), L-serine (SER), glucose (GLC), H 2 O 2 , KCl, and NaCl, were incubated with the EuMOG samples (3 mL) for 20 min.…”

“…Luminescent metal–organic frameworks (LMOFs), as a sub-field class of MOFs, have been proved as one of the most promising chemosensory materials owing to their intrinsic structure properties, unique luminescence features, and the fruitful energy/electron transfer processes among photonic units and analytes. − Till now, much efforts have been made on developing LMOFs for detection of various analytes − under different humidity/temperature conditions; − however, few studies on exploiting such materials for detecting α-KA have been reported. More importantly, most of the reported luminescent MOF-based sensors prefer working in the suspension state, which are subject to signal fluctuations because of the irregular movement of the insoluble MOF particles in the analytical sample.…”

Luminescent sensors based on coordination polymers with adjustable emissions for detecting biomarker of pollutant ethylbenzene and styrene

“…LCPs 1-3 were designed to probe PGA because the large overlap between the ultraviolet (UV) absorption spectrum of PGA and excitation spectra of LCPs 1-3 can give an intense competitive absorption of exciting energy and results in luminescence response to PGA. [18] Luminescent studies show that all the LCPs 1-3 exhibit significant sensing performance toward PGA. The K sv value and limit of detection (LOD) of LCP 2 with the best sensitivity are 4.06 ml mg −1 and 0.57 μg ml −1 , respectively.…”

Improving Covalent Organic Frameworks Fluorescence by Triethylamine Pinpoint Surgery as Selective Biomarker Sensor for Diabetes Mellitus Diagnosis

“…However, these techniques often require the intensive and time-consuming pretreatment procedure that generally can be carried out in well-equipped laboratories. Fluorescence sensing methods typically have a shorter processing time with less pretreatment of samples . Therefore, it is essential to develop simple and feasible fluorescence analysis to selectively monitor MGO in serum environment.…”