Concordância entre duas técnicas coproparasitológicas para diagnóstico de Fasciola hepatica em bovinos

Faria, Rodrigo; Cury, Márcia Cristina; Lima, W.S.

doi:10.1590/s0102-09352008000400037

Cited by 9 publications

(9 citation statements)

References 7 publications

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“…The protocol was implemented by using the FreeCalc software version 2 [(c) Copyright 2001-Angus Cameron AusVet Animal Health Services]. The sensitivity (92.7 %) and specificity (94.9 %) of the Flukefinder ® [ 26 ], 40 % minimum expected prevalence and cattle population interval of 1–250 animals were included as parameters. A maximum of 13 animals are required to be sampled in each household.…”

Section: Methodsmentioning

confidence: 99%

Cross-sectional study of Fasciola gigantica and other trematode infections of cattle in Edu Local Government Area, Kwara State, north-central Nigeria

et al. 2016

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BackgroundTrematode infections of livestock are of global veterinary and public health importance causing serious economic losses. Majority of data on burden of trematode infections in Nigeria are based on abattoir surveys and there are very few data on herd level risk factors. The present study investigated the prevalence of, and herd level risk factors for, fasciolosis and other trematode infections in cattle in Edu Local Government Area (LGA).MethodsA cross-sectional survey used two-stage study design to investigate cattle belonging to 65 households. Two questionnaires were administered for household-level and individual cattle-level data. Faecal and blood samples were obtained from the cattle. Logistic regression analyses were performed to determine risk factors for infections.ResultsOf 686 faecal samples analysed, 74.9 %, 16.1 %, 7.3 % and 1.2 % were positive for infections with Fasciola gigantica, paramphistomes, Dicrocoelium hospes and Schistosoma bovis respectively. Fasciola gigantica had higher prevalence in adult cattle (77.3 %) than weaners (62.5 %). Majority of co-infections was a combination of F. gigantica with paramphistomes 84/130 (64.6 %). Most (58.9 %) of the cattle belonged to FAMACHA© score 2. The mean packed cell volume (PCV) was 34.4 %. The sensitivity and specificity of FAMACHA© for anaemia (PCV < 24 %) were 18.2 and 96.9 %, respectively. Positive correlation was obtained between faecal egg counts for F. gigantica and paramphistomes (R = 0.15, P = 0.0001). Adult cattle were more likely to be infected with F. gigantica (odds ratio, OR: 1.94; Confidence Interval, CI: 1.19–3.16) than weaners. Cattle belonging to household heads aged between 40–59 years were more likely infected with paramphistomes (OR: 1.95; CI: 1.02–3.74) than those belonging to other age groups. Cattles from herds with size ≥ 100 were more likely infected with D. hospes than those from smaller herds (OR: 6.98; CI: 2.94–16.6).ConclusionThis study revealed high prevalence of infection with F. gigantica in Kwara State. The co-infections by F. gigantica and paramphistomes with a positive correlation should be considered during anthelmintic therapy. There is a need to optimise and validate the FAMACHA© for use in cattle based on breeds and variation in colour of ocular mucous membrane. Risk factors identified could assist in tailoring control strategies for various trematode infections to particular groups of farmers and cattle.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1737-5) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Cross-sectional study of Fasciola gigantica and other trematode infections of cattle in Edu Local Government Area, Kwara State, north-central Nigeria

et al. 2016

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“…14 Other coprological methods that hold promise as an alternative for Kato-Katz are the recently developed Mini-FLOTAC and Flukefinder test. Both methods have proven to have a high sensitivity for the diagnosis of fascioliasis in livestock, 15–17 but they have not yet been fully validated for the diagnosis of human fascioliasis. In this study, we compared the diagnostic performance of the standard method (Kato-Katz) with the recently developed methods Mini-FLOTAC and Flukefinder for the detection and quantification of F. hepatica eggs in human stool samples.…”

mentioning

confidence: 99%

“…The Kato-Katz thick smear (SterliTech, Kent, WA), 17 Flukefinder (Flukefinder, Soda Springs, ID), 16 and Mini-FLOTAC (Zinc Chloride flotation solution with a specific gravity of 1.30; University of Naples, Naples, Italy) 14 methods were performed according to the manufacturer’s instructions. The fecal egg counts (FECs; expressed in epg) were obtained by multiplying the egg counts by 24 for Kato-Katz (0.417 g of stool is examined), 10 for Mini-FLOTAC (0.1 g of stool is examined = 2 mL of stool suspension which contains 2 g in a total volume 40 mL), and 0.5 for Flukefinder (2 g of stool is examined).…”

mentioning

confidence: 99%

Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the Detection and Quantification of Fasciola hepatica Eggs in Artificially Spiked Human Stool

Zárate-Rendón

Vlaminck

Levecke

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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. We compared the diagnostic performance of the standard method (Kato-Katz) with two recently developed methods (Mini-FLOTAC and Flukefinder) for the detection and quantification of Fasciola hepatica eggs in human stool. Uninfected human stool samples were artificially spiked with F. hepatica eggs to reach final concentrations of 14, 28, 41, or 96 eggs per gram of stool (epg). Only Flukefinder showed 100% sensitivity in all but the samples with the lowest concentration of eggs (14 epg), in which it had a sensitivity of 60%. Each of the methods underestimated the true fecal egg counts (FECs), Flukefinder resulting in the most biased egg counts (egg counts 0.18 times lower than the expected FECs). Only the Flukefinder resulted in more precise results (coefficient of variance < 30%) from FECs of 96 epg onward. The outcome of this study indicates that the Flukefinder is a useful alternative diagnostic method for human fascioliasis in stool.

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“…Other authors compared the use of the Girão and Ueno [1], technique with a processing technique involving sequential filtration through two sieves of a Fluke finder unit and found similar results for the diagnosis of F. hepatica infection in cattle, but they advocate the use of the first technique because the materials for fabricating the Flukefinder device are imported, which limits the applicability of the test [12]. In the performance of the present study, the sedimentation technique described by Foreyt [2], was less costly than the Girão and Ueno [1], technique in addition to providing a higher percentage of egg retrieval.…”

Section: Resultsmentioning

confidence: 99%

“…However, the applicability must be taken into consideration in an attempt to reduce the costs and the time spent in preparing the tests without affecting the quality of the results [12].…”

Section: Resultsmentioning

confidence: 99%

Sedimentation technique (Foreyt, 2005) for quantitative diagnosis of Fasciola hepatica eggs.

Carneiro¹,

Martins²,

Avelar³

et al. 2018

J Parasitic Diseases

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Concordância entre duas técnicas coproparasitológicas para diagnóstico de Fasciola hepatica em bovinos

Cited by 9 publications

References 7 publications

Cross-sectional study of Fasciola gigantica and other trematode infections of cattle in Edu Local Government Area, Kwara State, north-central Nigeria

Cross-sectional study of Fasciola gigantica and other trematode infections of cattle in Edu Local Government Area, Kwara State, north-central Nigeria

Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the Detection and Quantification of Fasciola hepatica Eggs in Artificially Spiked Human Stool

Sedimentation technique (Foreyt, 2005) for quantitative diagnosis of Fasciola hepatica eggs.

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