2017
DOI: 10.7554/elife.28275
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Abstract: In Drosophila, graded expression of the maternal transcription factor Bicoid (Bcd) provides positional information to activate target genes at different positions along the anterior-posterior axis. We have measured the genome-wide binding profile of Bcd using ChIP-seq in embryos expressing single, uniform levels of Bcd protein, and grouped Bcd-bound targets into four classes based on occupancy at different concentrations. By measuring the biochemical affinity of target enhancers in these classes in vitro and g… Show more

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Cited by 79 publications
(117 citation statements)
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References 76 publications
(119 reference statements)
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“…Adding extra Zldbinding sites boosted the spatio-temporal response to the dorso-ventral gradient (Figure S1C-D). These temporal data are consistent with the idea that Zld potentiates the binding of spatially restricted morphogens like Bicoid or Dorsal 4,12,[16][17][18] , and thus supports the previously described pioneering activities of Zld 19,20 . Altogether we have set up a live imaging-based approach to quantitatively measure the impact of Zld on transcriptional dynamics in a developing dividing embryo.…”
Section: Zelda Fosters Temporal Transcriptional Coordinationsupporting
confidence: 91%
“…Adding extra Zldbinding sites boosted the spatio-temporal response to the dorso-ventral gradient (Figure S1C-D). These temporal data are consistent with the idea that Zld potentiates the binding of spatially restricted morphogens like Bicoid or Dorsal 4,12,[16][17][18] , and thus supports the previously described pioneering activities of Zld 19,20 . Altogether we have set up a live imaging-based approach to quantitatively measure the impact of Zld on transcriptional dynamics in a developing dividing embryo.…”
Section: Zelda Fosters Temporal Transcriptional Coordinationsupporting
confidence: 91%
“…We looked for the kinetic parameter set that yields the smallest positional resolution ∆X and, although the values vary with the interphase duration, we find that a parameter set that results in the experimentally observed ∆X data ∼ 12% EL in nc 12 does not change over multiple nuclear cycles of varying duration. This stability throughout the nuclear cycles is consistent with experimental observations that the Bcd interactions with the hb promoter are likely independent of other TF, which suggests the binding rate constant coefficients are independent of the nuclei's positions along the AP axes [43].…”
Section: Comparison To Experimental Datasupporting
confidence: 88%
“…Small (~20%) decreases in OTX2 in adult mouse visual cortex induce cortical plasticity (Spatazza et al, 2013;, and OTX2 transcriptional targets can be activated at one concentration of OTX2 yet be suppressed at lower or higher concentrations (Apulei et al, 2018). Concentration-dependent targets of homeoprotein are shown to be dependent on chromatin state, whereby some targets are concentration insensitive while others are highly concentration-sensitive (Hannon et al, 2017). Indeed, we find significant changes in some but not all putative targets of OTX2 in the V-SVZ despite the potentially subtle changes in OTX2 accumulation.…”
Section: Discussionmentioning
confidence: 99%