Concentration and Distribution of Mild and Severe Strains of Potato Spindle Tuber Viroid in Cross-Protected Tomato Plants

Khoury, Joseph; Singh, R. P.; Boucher, Alain; Coombs, D. H.

doi:10.1094/phyto-78-1331

Cited by 29 publications

(13 citation statements)

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“…However, infection of plants with the RNA-1 of tobacco rattle virus (TRV) is able to confer, in the absence of the RNA-2 encoded coat protein, protection towards infection with a complete TRV strain [ 1 ]. Additionally, the occurrence of cross-protection between viroids [ 11] indicates that proteins are not always involved.…”

Section: Discussionmentioning

confidence: 99%

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Wilk¹,

Willink²,

Huisman³

et al. 1991

Plant Mol Biol

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Transgenic potato plants, cultivar Désirée, were produced that contained the coat protein gene of potato leafroll luteovirus (PLRV). The transformed potato plants expressed the PLRV coat protein (CP) RNA sequences but accumulation of coat protein in transgenic tissues could not be detected. Upon inoculation with PLRV, the PLRV CP RNA expressing potato plants showed a reduced rate of virus multiplication.

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Section: Discussionmentioning

confidence: 99%

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Wilk¹,

Willink²,

Huisman³

et al. 1991

Plant Mol Biol

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“…R-PAGE (see below) was used to confirm the presence of TCDVd in subsequent plant inoculations. Cross-protection (Khoury et al ., 1988 ) was performed by pre-inoculating with PSTVd, followed by challenge inoculation with viroid from tomato.…”

Section: Methodsmentioning

confidence: 99%

Tomato chlorotic dwarf viroid: an evolutionary link in the origin of pospiviroids

Singh

Nie

Singh

1999

Journal of General Virology

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Over 40 isolates of potato spindle tuber viroid (PSTVd) have been reported from potato, other Solanum species and greenhouse tomato. These isolates have sequence similarities in the range 95-99 %. A viroid which caused chlorotic leaves and severe dwarfing of plants in greenhouse tomato crops was detected. The viroid was found to hybridize readily with PSTVd probes. It migrated faster than PSTVd in return-polyacrylamide gel electrophoresis and was not amplified in RT-PCR by a primer pair based on the lower strand of the central conserved region of PSTVd. Nucleotide sequencing of the viroid indicated that it is a circular RNA of 360 nt, with less than 90 % sequence similarities with PSTVd isolates. The Variable domain (V) has less than 60 % and the Terminal Right domain less than 90 % sequence similarity, while the remainder of the molecule has greater than 97 % similarity with PSTVd. Because of its less-than 90 % sequence similarities, unique V domain, lack of seed-transmission and lack of cross-protection by PSTVd, the viroid from tomato is proposed to be a distinct viroid species (tomato chlorotic dwarf viroid ; TCDVd) which also differs from two viroids infecting tomato in nature. TCDVd may be an evolutionary link in the development of crop viroids, with Mexican papita viroid as the ancestral viroid.

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“…It is noteworthy that this strain-specific degree of the severity of disease was virtually identical in the three tomato cultivars 'Sheyenne', 'Rutgers' and 'Rentita', irrespective of whether bioassaying was performed at Fredericton or Martinsried. Certain biological and electrophoretic properties of all these strains have been reported previously [8,20,[23][24][25]. The virulence modulating (VM) region [20], the conserved central region (CCR), and the variable region (VR) [6,7] of the viroid molecules are indicated.…”

mentioning

confidence: 98%

Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids

Herold

Haas

Singh³

et al. 1992

Plant Mol Biol

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The sequence analysis of five new field isolates of potato spindle tuber viroid (PSTVd) of different virulence revealed that the length of their RNA chain is not strictly conserved to 359 nucleotides (nts), as one could have inferred from the previously sequenced PSTVd strains. It was now found that the chain length is strain-specific like in the case of practically all other viroids, and that it may vary, so far, between 356 and 360 nts. Taking our previously sequenced and least virulent mild strain PSTVd KF6-M as standard, the new mild strains PSTVd WA-M and PSTVd F-M differ from it by one or two nts. The new intermediate-severe strains PSTVd F-IS and PSTV-F 88-IS differ from the standard mild strain by eight and nine nts, respectively, whereas the new severe-lethal strain PSTVd F-SL differs in seven nts. Most of these mutations are located within the virulence-modulating (VM) region and within the variable region (VR), and only in two strains a single mutation is found in the right terminal domain.

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Concentration and Distribution of Mild and Severe Strains of Potato Spindle Tuber Viroid in Cross-Protected Tomato Plants

Cited by 29 publications

References 0 publications

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Tomato chlorotic dwarf viroid: an evolutionary link in the origin of pospiviroids

Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids

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