Concentrated green tea supplement: Biological activity and molecular mechanisms

Cyboran, Sylwia; Strugała, Paulina; Włoch, Aleksandra; Oszmiański, Jan; Kleszczyńska, Halina

doi:10.1016/j.lfs.2014.12.025

Cited by 41 publications

(36 citation statements)

References 72 publications

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“…Erythrocyte membranes are known to have high cholesterol content, which may explain why there is no deeper interaction of EGCG with these membranes. Interestingly, EGCG was shown to increase the viscosity of pig ghost membranes when incubated in an isotonic buffer . The discrepancy found with our results may be explained by the differences in the buffer used, since it was already demonstrated that flavonoid membrane penetration is stimulated by the presence of salts and inhibited by the negative charges of the phospholipids .…”

Section: Discussioncontrasting

confidence: 99%

Differential inhibition of human erythrocyte acetylcholinesterase by polyphenols epigallocatechin‐3‐gallate and resveratrol. Relevance of the membrane‐bound form

et al. 2016

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The activity of acetylcholinesterase (AChE) from human erythrocytes was tested in the presence of the phenolic compounds resveratrol and epigallocatechin-3-gallate (EGCG). Even though the stilbene barely changed this enzymatic activity, EGCG did inhibit AChE. Importantly, it preferentially acted on the membrane-bound enzyme rather than on its soluble form. Actually, it was shown that this flavonoid may bind to the red blood cell membrane surface, which may improve the interaction between EGCG and AChE. Therefore, caution should be taken when screening AChE inhibitors. In fact, testing compounds with the soluble form of the enzyme may underestimate the activity of some of these potential inhibitors, hence it would be advisable not to use them as a sole model system for screening. Moreover, erythrocyte AChE is proposed as a good model for these enzymatic assays. © 2016 BioFactors, 43(1):73-81, 2017.

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Section: Discussioncontrasting

confidence: 99%

Differential inhibition of human erythrocyte acetylcholinesterase by polyphenols epigallocatechin‐3‐gallate and resveratrol. Relevance of the membrane‐bound form

et al. 2016

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“…Taking in consideration the study of Cyboran et al (2015) 8 , that evidenced that polyphenols extracted from green tea had effect on enzymes involved in inflammation such as COX-1 (cyclooxygenase-1), COX-2 (cyclooxygenase-2) and lipoxygenase, we can suppose that these enzymes are the targets of green tea extract [8].…”

Section: Discussionmentioning

confidence: 99%

Atividade citotóxica e anti-inflamatória do extrato glicólico de Camellia sinensis (L.) Kuntze em macrófagos (RAW 264.7) estimulados por LPS

Higa¹,

Jorjão

Lapena

et al. 2020

BJHR

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Atividade citotóxica e anti-inflamatória do extrato glicólico de Camellia sinensis (L.) Kuntze em macrófagos (RAW 264.7) estimulados por LPS / Cytotoxic and antiinflamatory activity of the glycolic extract of Camellia sinensis (L.) Kuntze in LPS-stimulated machrophages (RAW 264.7) ABSTRACT Plant extracts can be a source of diverse biologic activities, as anti-inflammatory action, which is an interesting characteristic for mouthwashes, toothpastes and intracanal medication. This study aimed to evaluate cytotoxic and anti-inflammatory activity of Camellia sinensis (L.) Kuntze (green tea) glycolic extract in mouse macrophages (RAW 264.7). Cytotoxic activity was measured by the metabolic activity of MTT test, macrophages were distributed in 96 wells and exposed to 11 serial dilutions of each extract (200 mg/mL to 0.20 mg/mL). After 5 min and 24 h of contact, cell viability was assessed. Then, the extract at concentrations of 3.13 mg / mL and 12.5 mg / mL, were chosen to verify antiinflammatory activity. For this, after exposure time of 5 min or 24 h to the extract, supernatants of LPS-stimulated RAW 264.7 cultures were collected to quantify pro-inflammatory cytokines IL-1β and TNF-α by immunoenzymatic test (ELISA). The results were evaluated with statistical analyses (ANOVA and Turkey test), with p ≤ 0.05. Results: In MTT test green tea promoted increase cell viability in all concentrations at 5 min. The cell viability was greater than 100% in concentration of 0,20mg/mL to 12,5 mg/mL, at the time of exposure of 24h. The concentration of 12.5 mg/mL was the highest concentration less cytotoxic. The extract showed anti-inflammatory potential evidenced by the production decrease of IL-1β and TNF-α with better results at a concentration of 12.5 mg/mL 1096 for both exposure times. These results indicate promising immunomodulatory features of green tea. Therefore, this plant extract showed to be an interesting alternative to be inserted in medical or oral products or even as a source of active compounds. RESUMOExtratos de plantas podem ser fonte de diversas atividades biológicas. Dentre essas, a ação antiinflamatória se apresenta como uma das características de interesse para enxaguantes bucais, pastas dentais e medicações intracanais. O objetivo deste estudo foi avaliar a atividade citotóxica e antiinflamatória do extrato glicólico de Camellia sinensis (L.) Kuntze (chá verde) em macrófagos de camundongos (RAW 264.7). A atividade citotóxica foi medida pela atividade metabólica do teste de MTT, os macrófagos foram distribuídos em 96 poços e expostos a 11 diluições seriadas de cada extrato (200 mg / mL a 0,20 mg / mL). Após 5 min e 24 h de contato, a viabilidade celular foi avaliada. Então, o extrato nas concentrações de 3,13mg/mL e 12,5 mg / mL , foram escolhidas para verificar a atividade anti-inflamatória. Para isso, após a exposição do extrato por 5 minutos ou 24 horas, os sobrenadantes das células RAW 264.7 foram coletados para quantificação das citocinas pró-inflamatórias IL-1β e TNF-α pelo teste imunoenzimático ELISA...

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“…Changes of plasma antioxidant activity in response to repeated strawberry polyphenols ingestion could be the net result of several processes including for instance: (A) – elaboration of polyphenols by gut microflora and intestinal absorption; (24,37,38) (B) – formation of less reactive complexes with plasma proteins and their transportation outside the capillaries; (39,40) (C) – binding and uptake of polyphenols by blood and endothelial cells; (41,42) (D) – polyphenols metabolism in liver and their urinary excretion. (43) Moreover, some of these processes can be activated or inhibited by polyphenols themselves.…”

Section: Discussionmentioning

confidence: 99%

Addition of strawberries to the usual diet increases postprandial but not fasting non-urate plasma antioxidant activity in healthy subjects

Prymont-Przymińska

Białasiewicz

Zwolinska

et al. 2016

J. Clin. Biochem. Nutr.

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Strawberries can augment plasma antioxidant activity, but this may be confounded by selection of methods, time of blood sampling and concomitant dietary restrictions. We examined the effect of strawberry consumption on ferric reducing ability (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (DPPH-test) of native and non-urate plasma in healthy subjects on their usual diet. Eleven subjects consumed strawberries (500 g daily) for 9 days. Fasting and 3-h postprandial plasma and 24-h urine collection were obtained before, during and after strawberry course for FRAP, DPPH-test and polyphenols determination. Fifteen subjects served as a control in respect to plasma antioxidant activity changes and effect of 300 mg of oral ascorbate. First, 5th and 9th strawberry dose increased 3-h postprandial DPPH-test by 17.4, 17.6 and 12.6%, and FRAP by 15.5, 25.6 and 21.4% in comparison to fasting values in non-urate plasma (p<0.05). In native plasma only a trend was observed to higher postprandial values for both tests. Strawberries increased urinary urolithin A and 4-hydroxyhippuric acid whereas plasma polyphenols were stable. No changes of FRAP and DPPH-test were noted in controls and after ascorbate intake. Strawberries transiently increased non-urate plasma antioxidant activity but this cannot be attributed to direct antioxidant effect of polyphenols and ascorbate.

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Concentrated green tea supplement: Biological activity and molecular mechanisms

Cited by 41 publications

References 72 publications

Differential inhibition of human erythrocyte acetylcholinesterase by polyphenols epigallocatechin‐3‐gallate and resveratrol. Relevance of the membrane‐bound form

Differential inhibition of human erythrocyte acetylcholinesterase by polyphenols epigallocatechin‐3‐gallate and resveratrol. Relevance of the membrane‐bound form

Atividade citotóxica e anti-inflamatória do extrato glicólico de Camellia sinensis (L.) Kuntze em macrófagos (RAW 264.7) estimulados por LPS

Addition of strawberries to the usual diet increases postprandial but not fasting non-urate plasma antioxidant activity in healthy subjects

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