Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.11.020 View full text
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Paul G. Stevenson, Abhijit Tarafder, Georges Guiochon

Abstract: A 2D comprehensive chromatographic separation of blackberry sage fragrant oil was performed by using HPLC in the first dimension and SFC in the second. A C(18)-bonded silica column eluted with an ACN gradient was used in the HPLC dimension and an amino-bonded silica column eluted with ACN as a modifier in the SFC dimension. This 2D separation was completed in the off-line mode, the fractions from the HPLC column being collected and injected in the SFC column. The retention factors on the two columns have a -0.…

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