Comprehensive temporal reprogramming ensures dynamicity of transcriptomic profile for adaptive response in Taxus contorta

Majeed, Aasim; Singh, Amandeep; Sharma, Ram Kumar; Jaitak, Vikas; Bhardwaj, Pradeep

doi:10.1007/s00438-020-01709-2

Cited by 10 publications

(2 citation statements)

References 63 publications

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“…The signal patterns on the chromosomes of the five species of Taxus are different, which we believe may be because the plant adapts to environmental disturbances and changes the gene expression profile to adapt to the environmental change [ 62 ], resulting in more gene deletion and acquisition. However, genomic studies are hampered by the lack of reference genomes and the transcriptome of non-model plants, so the genomic level of gymnosperms is still being explored [ 63 ]. Of course, the more plausible reasons need to be further studied.…”

Section: Discussionmentioning

confidence: 99%

Five Species of Taxus Karyotype Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Luo

Lei

et al. 2022

Genes

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As a relict plant, Taxus is used in a variety of medicinal ingredients, for instance to treat a variety of cancers. Taxus plants are difficult to distinguish from one another due to their similar morphology; indeed, some species of Taxus cytogenetic data still are unclear. Oligo-FISH can rapidly and efficiently provide insight into the genetic composition and karyotype. This is important for understanding the organization and evolution of chromosomes in Taxus species. We analysed five Taxus species using two oligonucleotide probes. (AG3T3)3 signals were distributed at the chromosome ends and the centromere of five species of Taxus. The 5S rDNA signal was displayed on two chromosomes of five species of Taxus. In addition to Taxus wallichiana var. mairei, 5S rDNA signals were found proximal in the remaining four species, which signals a difference in its location. The karyotype formula of Taxus wallichiana was 2n = 2x = 24m, its karyotype asymmetry index was 55.56%, and its arm ratio was 3.0087. Taxus × media’s karyotype formula was 2n = 2x = 24m, its karyotype asymmetry index was 55.09%, and its arm ratio was 3.4198. The karyotype formula of Taxus yunnanensis was 2n = 2x = 24m, its karyotype asymmetry index was 55.56%, and its arm ratio was 2.6402. The karyotype formula of Taxus cuspidate was 2n = 2x = 24m, its karyotype asymmetry index was 54.67%, its arm ratio was 3.0135, and two chromosomes exhibited the 5S rDNA signal. The karyotype formula of T. wallichiana var. mairei was 2n= 2x = 22m + 2sm, its karyotype asymmetry index was 54.33%, and its arm ratio was 2.8716. Our results provide the karyotype analysis and physical genetic map of five species of Taxus, which contributes to providing molecular cytogenetics data for Taxus.

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Section: Discussionmentioning

confidence: 99%

Five Species of Taxus Karyotype Based on Oligo-FISH for 5S rDNA and (AG3T3)3

Luo

Lei

et al. 2022

Genes

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“…However, plants exposed to high light for a long time produce ROS, thus affecting JA biosynthesis or signaling. It has been suggested that the JA signaling pathway is closely related to paclitaxel biosynthesis in Taxus [ 23 , 26 , 40 ]. Xiong et al reported that JA treatment could promote CYP450 gene expression, which was related to paclitaxel biosynthesis and could enhance the baccatin III and paclitaxel content in the Taxus cell line [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis provides insights into light condition effect on paclitaxel biosynthesis in yew saplings

Liao

et al. 2022

BMC Plant Biol

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Background Taxus is a rare gymnosperm plant that is the sole producer of the anticancer drug paclitaxel. The growth and development of Taxus is affected by environmental factors such as light. However, little is known about how light conditions affect growth and metabolic processes, especially paclitaxel biosynthesis. Results In this study, we applied three different light conditions to Taxus chinensis young saplings and investigated the physiological response and gene expression. Our observations showed that exposure to high light led to oxidative stress, caused photoinhibition, and damaged the photosynthetic systems in T. chinensis. The paclitaxel content in T. chinensis leaves was significantly decreased after the light intensity increased. Transcriptomic analysis revealed that numerous genes involved in paclitaxel biosynthesis and phenylpropanoid metabolic pathways were downregulated under high light. We also analyzed the expression of JA signaling genes, bHLH, MYB, AP2/ERF transcription factors, and the CYP450 families that are potentially related to paclitaxel biosynthesis. We found that several CYP450s, MYB and AP2/ERF genes were induced by high light. These genes may play an important role in tolerance to excessive light or heat stress in T. chinensis. Conclusions Our study elucidates the molecular mechanism of the effects of light conditions on the growth and development of T. chinensis and paclitaxel biosynthesis, thus facilitating the artificial regeneration of Taxus and enhancing paclitaxel production.

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