2023
DOI: 10.1007/978-1-0716-3183-6_9
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Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications

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“…However, ChIP assay and the deriving data are still the primary method to investigate protein–chromatin interactions, at least in model species, also in reference to stress-related contexts. For example, in a very recent study, publicly available ChIP-seq datasets of Arabidopsis wild-type and mutants defective in key enzymes of histone modification and chromatin remodelling were used to produce a comprehensive list of iron homeostasis genes with differential enrichment of various histone modifications [ 40 ]. Furthermore, novel procedures are being developed to overcome some of the limitations, such as the relatively high amount of plant material needed for chromatin extraction and immunoprecipitation [ 41 ].…”
Section: Plant Epigenetic Mechanisms: How Do They Work and How Can We...mentioning
confidence: 99%
“…However, ChIP assay and the deriving data are still the primary method to investigate protein–chromatin interactions, at least in model species, also in reference to stress-related contexts. For example, in a very recent study, publicly available ChIP-seq datasets of Arabidopsis wild-type and mutants defective in key enzymes of histone modification and chromatin remodelling were used to produce a comprehensive list of iron homeostasis genes with differential enrichment of various histone modifications [ 40 ]. Furthermore, novel procedures are being developed to overcome some of the limitations, such as the relatively high amount of plant material needed for chromatin extraction and immunoprecipitation [ 41 ].…”
Section: Plant Epigenetic Mechanisms: How Do They Work and How Can We...mentioning
confidence: 99%