Comprehensive and quantitative stability study of ascorbic acid using capillary zone electrophoresis with ultraviolet detection and high‐resolution tandem mass spectrometry

Abstract

Ascorbic acid is a powerful antioxidant compound involved in many biological functions, and a chronic deficiency is at the origin of scurvy disease. A simple, rapid, and cost‐effective capillary electrophoresis method was developed for the separation and simultaneous quantification of ascorbic acid and the major degradation products: dehydroascorbic acid, furfural, and furoic acid. Systematic optimization of the conditions was performed that enabled baseline separation of the compounds in less than 10 min. In addition to simultaneous quantification of ascorbic acid alongside to the degradation products, stability studies demonstrated the possibility using capillary electrophoresis to separate and identify the major degradation products. Thus, high‐resolution tandem mass spectrometry experiments were conducted in order to identify an unknown degradation product separated by capillary electrophoresis and significantly present in degraded samples. Comparison of mass spectrometry data and capillary electrophoresis electropherograms allowed to identify unambiguously trihydroxy‐keto‐valeraldehyde. Finally, capillary electrophoresis was successfully applied to evaluate the composition of different pharmaceutical preparation of ascorbic acid. Results showed the excellent performance of the capillary electrophoresis method due to the separation of excipients from the compounds of interest, which demonstrated the relevance of using an electrophoretic separation in order to perform comprehensive stability studies of ascorbic acid.

Molecular Separation by Using Active and Passive Microfluidic chip Designs: A Comprehensive Review

“…Free zone electrophoresis was originally based on methods for purifying cells and bacteria. Zone electrophoresis (ZE), by default, is an appropriate method for separating [27] small molecules, proteins, [281,282] biomolecules, [283] peptides, [217,284] DNA, [285,286] viruses, [218,287] and membranes. In capillary zone electrophoresis (CZE), the electrophoretic separation of glycoproteins and glycopeptides can be performed by consideration of the m and z differences among the input ingredients, where m is the mass and z is the charge of the compartments.…”

Non‐Enzymatic Electrochemical Biosensor for Dopamine Detection Using MoS2/rGO/Ag Nanostructure

“…Methods like HPLC, [6] HPLC-VWD, [7] chemiluminescence, [8] capillary electrophoresis, [9] Ultraviolet Detection and High-Resolution Tandem Mass Spectrometry techniques have found extensive use for the selective determination of DA. [10] Apart from all these techniques, electrochemical biosensors have emerged as one significant technique for DA sensing in recent times. These are noninvasive with low detection limit, are cost competent, highly sensitive, stable and selective.…”

“…Hence, early detection of DA levels in the body fluid becomes crucial. Methods like HPLC, [6] HPLC‐VWD, [7] chemiluminescence, [8] capillary electrophoresis, [9] Ultraviolet Detection and High‐Resolution Tandem Mass Spectrometry techniques have found extensive use for the selective determination of DA [10] . Apart from all these techniques, electrochemical biosensors have emerged as one significant technique for DA sensing in recent times.…”