Compounds Released by the Biocontrol Yeast Hanseniaspora opuntiae Protect Plants Against Corynespora cassiicola and Botrytis cinerea

Abstract

Plant diseases induced by fungi are among the most important limiting factors during pre- and post-harvest food production. For decades, synthetic chemical fungicides have been used to control these diseases, however, increase on worldwide regulatory policies and the demand to reduce their application, have led to searching for new ecofriendly alternatives such as the biostimulants. The commercial application of yeasts as biocontrol agents, has shown low efficacy compared to synthetic fungicides, mostly due to the limited knowledge of the molecular mechanisms of yeast-induced responses. To date, only two genome-wide transcriptomic analyses have characterized the mode of action of biocontrols using the plant model Arabidopsis thaliana, missing, in our point of view, all its molecular and genomic potential. Here we describe that compounds released by the biocontrol yeast Hanseniaspora opuntiae (HoFs) can protect Glycine max and Arabidopsis thaliana plants against the broad host-range necrotrophic fungi Corynespora cassiicola and Botrytis cinerea. We show that HoFs have a long-lasting, dose-dependent local, and systemic effect against Botrytis cinerea. Additionally, we performed a genome-wide transcriptomic analysis to identify genes differentially expressed after application of HoFs in Arabidopsis thaliana. Our work provides novel and valuable information that can help researchers to improve HoFs efficacy in order for it to become an ecofriendly alternative to synthetic fungicides.

Plant evolution and environmental adaptation unveiled by long-read whole-genome sequencing ofSpirodela

“…RNA-Seq for Arabidopsis (accession no. GSM3120107) (61) and for Spirodela (accession no. PRJNA557001).…”

Insight into the Microbiological Control Strategies against Botrytis cinerea Using Systemic Plant Resistance Activation

“…In this sense, the abilities of different yeasts to activate systemic plant resistance against the necrotrophic pathogen have been described. All the studies carried out to date have used A. thaliana as a model plant, reporting a significant in the systemic expression of JA/ET-related genes, such as ACS6, PR4, and PDF1.2, after the application of yeasts, such as Hanseniaspora opuntiae and Pseudozyma aphidis, on leaves [113,115]. The plant response elicited by the components of the fungal cell wall, like that under the foliar application of autoclaved S. cerevisiae cells, increases the systemic expression of PR genes and the accumulation of the phytoalexin camalexin via the SA-pathway [119].…”

Effects of yeast fermentation broths on the Meloidogyne incognita population in soybean

“…P. guilliermondii induced systemic resistance in tomatoes infected with M. incognita and increased defense enzymes such as peroxidase and polyphenol oxidase (Hashem and Abo-Elyousr 2011). H. opuntiae filtrates can induce local and systemic protective mechanisms in Arabidopsis thaliana (L.) Heynh against Botrytis cinerea (de Bary) Whetze that involve the jasmonic acid and ethylene pathways (Ferreira-Saab et al 2018).…”

“…Yeasts can be used as biocontrol agents against plant pathogens due to their fast multiplication, capacity to produce antibiotic and lytic enzymes, ability to compete for nutrients and space and to induce resistance in the host, and involvement in oxidative stress response and in the production of growth regulators (El-Tarabily and Sivasithamparam 2006;Spadaro and Droby 2016). Yeast byproducts, such as yeast filtrates, also have the potential to activate resistance mechanisms (Stangarlin et al 2010;Ferreira-Saab et al 2018) in addition to having a direct toxic effect on pathogens (Hashem et al 2008;Regaieg et al 2010). Nevertheless, research on the use of yeasts and their byproducts in nematode management remains scarce.…”