Sourdough fermentation of bran can overcome the technological problems encountered in bread making but favors the activity of endogenous β-glucanase leading to a degradation of β-glucans. This study investigated the effects of high-intensity ultrasound (US) pretreatment of oat (OB) and barley (BB) bran on its β-glucans content, properties, and preservation in processing, as well as the on the acidification kinetics of bran sourdough fermentation and on its application in flatbread. To reduce β-glucanase activity, OB and BB (15 % water suspensions) were US-pretreated prior to sourdough fermentation. The acidification kinetics, the microbial viable cell count, and the total titratable acidity (TTA) of the sourdough were determined. The total β-glucans content of bran, sourdough, and bread, as well as water solubility and the molecular weight (Mw) of control and US-pretreated bran were investigated. The physical properties of control wheat and composite flatbreads were compared. The US-pretreatment increased the acidification rate (30%) and TTA (51%) of OB sourdough, however decreased the acidification rate of BB (18%). After the US-pretreatment of OB and BB, the total (11.5-12.3 %) and water-soluble β-glucans (31-40 %) increased while their Mw decreased (7-21.7 %). In sourdough and flatbread prepared with US-pretreated OB/BB, 93-95 % and 90-98 % of β-glucans were retained, respectively, compared to 64-72 % and 82-92 % in control samples. The US-pretreatment and/or sourdough fermentation of OB and BB resulted in flatbreads of higher specific volume (8-22 %) and cohesiveness (11-20%) while reduced hardness (40-55 %) and chewiness (51-73 %) compared to their control bread.